职称 教授

学位 博士

电话 027-87286952

邮箱 lilin@mail.hzau.edu.cn

研究方向 假单胞菌功能基因与生物技术

 

教育经历

1995.09-1999.07，华中农业大学，理学博士

1990.09-1993.07，南京大学，理学硕士

1982.09-1986.07，湖北大学，理学学士

 

主要职历

1993.07-现在，华中农业大学生命科学技术学院，教授

2019.01-2019.02，美国加州大学戴维斯分校，访问学者

2000.07-2002.07，韩国Pohang University of Science and Technology，博士后

2003.01-2004.12，德国Max-Planck-Institut fuer InfektionsBiologie，博士后

1986.07-1990.09，湖北文理学院化工系，助教

 

科研成果

科研方向

主要从事丁香假单胞菌、恶臭假单胞菌与杀虫、致病、环境降解相关的功能基因、表达调控、作用机制与应用技术基础等方面的研究工作。主要科研方向包括：（1）丁香假单胞菌杀线虫功能基因、活性调控与作用机制；（2）假单胞菌降解环境难降解和有毒化合物的功能基因、降解活性、作用途径与机制；（3）锰氧化假单胞菌活性、生物锰氧化物表征与应用。

 

学术成绩

长期以来主要围绕丁香假单胞菌冻害相关的冰核基因活性与功能应用、丁香假单胞菌杀线虫功能基因、活性调控与作用机制、以及假单胞菌与芽孢杆菌（包括重组菌株）在环境激素、有机农药和染料降解脱色等方面进行了一系列研究工作，发表SCI论文50余篇，其它学术论文30余篇，参与撰写英文学术专著2部，中文学术专著2部，获批国家发明专利5项。

 

主持科研项目

1. 国家自然科学基金，利用II型CRISPR-Cas9系统改造锰氧化假单胞菌T34及用于生物模板法构建高势能电极材料的研究（31770108），2018/12-2021/12，60万元

2. 国家自然科学基金，丁香假单胞菌GntR/HutC类转录调控因子InpR调控网络解析与调控冰核基因分子机制研究（31570123），2016/01-2019/12，65万元，已结题

3. 国家重点基础研究计划（973计划）课题，细菌侵染线虫的分子机制（2013CB127504），2013/01-2017/08，590万元，已结题

4. 湖北省公益性科技研究项目，广谱高效的苏云金芽孢杆菌纳米工程菌制剂的研制与应用技术研究（2012DBA10001），2013/01-2014/12，10万元，已结题

5. 国家自然科学基金，土壤细菌生物氧化Mn(II)的分子机制研究（31270158），2013/01-2016/12，78万元，已结题

6. 国家自然科学基金，丁香假单胞菌冰核蛋白跨膜分泌效应分子的研究（31070111），2011/01-2013/12，34万元，已结题

7. 国家高技术研究发展计划（863计划），基于组学分析和代谢工程技术分析的苏云金杆菌高毒力菌种的选育（2008AA02Z112），2018/01-2010/12，98万元，已结题

 

代表性学术论著

1. Anum Bashir, Tian Tian, Xun Yu, Cui Meng, Muhammad Ali, Lin Li*. Pyoverdine-mediated killing of Caenorhabditis elegans by Pseudomonas syringae MB03 and the role of iron in its pathogenicity. International Journal of Molecular Sciences 2020, 21:2198. (通讯作者)

2. Zhen Zhang, Zhiyong Ruan, Jin Liu, Chang Liu, Fuming Zhang, Robert J. Linhardt, Lin Li*. Complete degradation of bisphenol A and nonylphenol by a composite of biogenic managanese oxides and Escherichia coli cells with surface-displayed multicopper oxidase CotA. Chemical Engineering Journal 2019, 362: 897-908. (通讯作者)

3. Zhen Zhang, Jin Liu, Jin Fan, Zhiyong Wang, Lin Li*. Detection of catechol using an electrochemical biosensor based on engineered Escherichia coli cells that surface-display laccase. Analytica Chimica Acta 2018, 1009: 65-72. (通讯作者)

4. Abdul Manan, Zahoor Ahmad Bazai, Jin Fan, Huafu Yu, Lin Li*. The Nif3-family protein YqfO03 from Pseudomonas syringae MB03 has multiple nematicidal activities against Caenorhabditis elegans and Meloidogyne incognita. International Journal of Molecular Sciences 2018, 19, 3915. (通讯作者)

5. Zhiyong Wang, Jieping Wang, Jin Liu, Hong Chen, Mingshun Li, Lin Li*. Mechanistic insights into manganese oxidation of a soil-borne Mn(II)-oxidizing Escherichia coli strain by global proteomic and genetic analyses. Scientific Reports 2017, 7: 1352(通讯作者)

6. Penggao Hu, Zhongna Zhang, Fei Shen, Xun Yu, Mingshun Li, Hong Ni, Lin Li*. Poly-g-glutamic acid coupled Pseudomonas putida cells surface-displaying metallothioneins: composited copper(II) biosorption and inducible flocculation in aqueous solution. RSC Advances 2017, 7: 18578-18587.(通讯作者)

7. Lin Li*, Zhenmin Chen, Ziniu Yu. Mass production, application, and market development of Bacillus thuringiensis biopesticides in China. In: Bacillus thuringiensis and Lysinibacillus sphaericus, Fiuza LM et al (eds.), Springer International Publishing AG 2017. ISBN: 978-3-319-56677-1. pp. 185-212, 2017. DOI: 10.1007/978-3-319-56678-8_12.(通讯作者)

8. Xu Qin, Xuemei Xiang, Xiaowen Sun, Hong Ni, Lin Li*. Preparation of nanoscale Bacillus thuringiensis chitinases using silica nanoparticles for nematicide delivery. International Journal of Biological Macromolecules 2016, 82: 13-21. (通讯作者)

9. Hua Li, Penggao Hu, Xiuyun Zhao, Ziniu Yu, Lin Li*. Bacillus thuringiensis peptidoglycan hydrolase SleB171 involved in daughter cell separation during cell division. Acta Biochimica et Biophysica Sinica 2016, 48(4): 354-362. (通讯作者)

10. Muhammad Ali, Yu Sun, Li Xie, Huafu Yu, Anum Bashir, Lin Li*. The pathogenicity of Pseudomonas syringae MB03 against Caenorhabditis elegans and the transcriptional response of nematicidal genes upon different nutritional conditions. Frontiers in Microbiology 2016, 7: 805. (通讯作者)

11. Jin Liu, Luming Tan, Jing Wang, Zhiyong Wang, Hong Ni, Lin Li*. Complete biodegradation of chlorpyrifos by engineered Pseudomonas putida cells expressing surface-immobilized laccases. Chemosphere 2016, 157: 200-207. (通讯作者)

12. Zhen Zhang, Zhongming Zhang, Hong Chen, Jin Liu, Chang Liu, Hong Ni, Changsong Zhao, Muhammad Ali, Fan Liu, Lin Li*. Surface Mn(II) oxidation actuated by a multicopper oxidase in a soil bacterium leads to the formation of manganese oxide minerals. Scientific Reports 2015, 5: 10895 (通讯作者)

13. Hong Ni, Siquan Zeng, Xu Qin, Xiaowen Sun, Shan Zhang, Xiuyun Zhao, Ziniu Yu, Lin Li*. Molecular docking and site-directed mutagenesis of a Bacillus thuringiensis chitinase to improve chitinolytic, synergistic lepidopteran-larvicidal and nematicidal activities. International Journal of Biological Sciences 2015, 11(3): 304-315. (通讯作者)

14. Hong Ni, Zhe Xiong, Ting Ye, Zhen Zhang, Xiaofeng Ma, Lin Li*. Biosorption of copper(II) from aqueous solutions using volcanic rock matrix-immobilized Pseudomonas putida cells with surface-displayed cyanobacterial metallothioneins. Chemical Engineering Journal 2012, 204-205: 264-271. (通讯作者)

15. Wei Wang, Zhen Zhang, Hong Ni, Xiaomeng Yang, Qianqian Li, and Lin Li*. Decolorization of industrial synthetic dyes using engineered Pseudomonas putida cells with surface-immobilized bacterial laccase. Microbial Cell Factories 2012, 11: 75. (通讯作者)

16. Qianqian Li, Qi Yan, Jinsi Chen, Yan He, Jing Wang, Hongxing Zhang, Ziniu Yu, and Lin Li*. Molecular characterization of an ice nucleation protein variant (InaQ) from Pseudomonas syringae and the analysis of its transmembrane transport activity in Escherichia coli. International Journal of Biological Sciences 2012; 8(8):1097-1108.(通讯作者)

17. Xiaohu Shao, Hong Ni, Ting LU, Mengtian Jiang, Hua Li, Xinfeng Huang, Lin Li*. An improved system for the surface immobilisation of proteins on Bacillus thuringiensis vegetative cells and spores through a new spore cortex-lytic enzyme anchor. New Biotechnology 2012, 29(3): 302-310.(通讯作者)

18. Lin Li*, Ziniu Yu. Genetically Modified Bacillus thuringiensis Biopesticides. In: Bacillus thuringiensis Biotechnology. Ed. Estibaliz Sansinenea. Springer publishing, 2012. pp. 231-258. ISBN: 978-94-007-3020-5. DOI: 10.1007/978-94-007-3021-2. (通讯作者)

 

授权发明专利

1. 李林，谭璐明，王智勇，张震. 一种锰氧化假单胞菌T34及生物氧化锰制备方法和降解环丙沙星的应用. 国家发明专利, 专利号: ZL 201410387871.3.

2. 李林，汤浪，白志强, 刘瑾. 一种利用壳聚糖为载体制备的生物复合材料及应用.国家发明专利, 专利号: 201510195759.4

3. 李林，向雪梅. 一种纳米Bt复配杀虫剂及制备方法与应用.国家发明专利, 专利号: ZL 201410189514.6.

4. 李林，秦旭，孙孝文，顾桐，张震. 一种丁香假单胞菌及其筛选方法和在杀灭线虫中的应用.国家发明专利, 专利号: ZL201410199803.4

5. 李林，白志强，汤浪，张震. 一种壳聚糖珠的制备方法及其制备装置.国家发明专利, 专利号: ZL 201310741611.7