职称 教授

学位 博士

政治面貌 中共党员

电话 027-87644219

邮箱 lzf6789@mail.hzau.edu.cn

研究方向 动物传染病和人畜共患病

教育经历

1997.09-2002.07，华中农业大学，兽医分子抗病育种博士

1993.09-1997.07，华中农业大学，畜牧专业学士

主要职历

2011.11-现在，华中农业大学动科-动医学院，教授

2007.04-2011.10，华中农业大学动科-动医学院，副教授

2004.10-2007.04，美国堪萨斯州立大学（Kansas State University），博士后

2002.07-2004.09，华中农业大学动科-动医学院，讲师

科研成果

科研方向

作为农业微生物学国家重点实验室固定研究人员，湖北省“农业动植物病虫害防治的微生物学研究”优秀创新团队骨干成员，主要从事动物传染病和人畜共患病的教学、科研和开发工作。具体包括：（1）α疱疹病毒(包括PRV、BoHV-1、BoHV-5)：对α疱疹病毒研究试图弄清其潜伏感染以及嗜神经性机理；（2）布鲁氏菌：布鲁氏菌（Brucella）是严重危害人畜健康的动物源性人兽共患病的病原体,对羊、牛和猪等造成巨大的经济损失。揭示其免疫逃逸及胞内寄生机制将为新型疫苗和药物的研制提供理论基础；（3）新型疫苗和诊断试剂（盒）研究开发。

学术成绩

研制新型疫苗4个，诊断试剂2种，获发明专利5项，湖北省科技进步一等奖1项，优秀研究生论文指导奖4项，教学质量优秀奖4项。以第一作者或通讯作者在Nucleic Acids Research、Journal of Virology和《微生物学报》等国内外刊物上发表论文31篇。

主持科研项目

1. 国家重点研发计划“新发与再现畜禽重大疫病的致病与免疫机制研究”课题5“猪伪狂犬病毒潜伏感染及其致病机制”（编号：2016YFD0500105），625万，2016.7-2020.12

2. 国家自然科学基金面上项目“伪狂犬病毒lncRNA（CTO）对病毒复制和潜伏感染的调控作用研究”（编号：31770191），直接经费65万，2018.1-2021.12

代表性学术论文

1. Wang Ji, Wang Cheng, Xu Na, Liu Zheng-Fei*, Pang Dai-Wen, Zhang Zhi-Ling**. A virus-induced kidney disease model based on organ-on-a-chip: pathogenesis exploration of virus-related renal dysfunctions. Biomaterials. 2019, 219: 119367 (IF=10.273) （共同通讯作者）

2. Yan Kai, Liu Jie, Guan Xiang, Yin Yi-Xin, Peng Hui, Chen Huanchun, and Liu Zheng-Fei*. The carboxyl terminus of tegument protein pUL21 contributes to pseudorabies virus neuroinvasion. Journal of Virology. 2019, 93(7): 02052.18. (IF= 4.368)

3. Liu Fang, Zheng Ke, Chen Huan-Chun, Liu Zheng-Fei*.Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes. Nucleic Acids Research. 2018, 46(21): e129 (IF= 11.147)

4. Guan Xiang, Liu Jie, Jiang Hui, Wu Chang-Xian, Chen Huan-Chun, Liu Zheng-Fei*. Expression of pseudorabies virus-encoded long noncoding RNAs in epithelial cells and neurons. Journal of NeuroVirology. 2018, 24(5): 597–605 (IF= 3.228)

5. Wang Xin, Zhang Mei-Mei, Yan Kai, Tang Qi, Wu Yi-Quan, He Wen-Bo, Chen Huan-Chun, Liu Zheng-Fei*. The full-length microRNA cluster in the intron of large latency transcript is associated with the virulence of pseudorabies virus. Virology. 2018, 520:59-66 (IF= 3.374)

6. Zheng Ke, Wang Yang, Li Na, Jiang Fang-Fang, Wu Chang-Xian, Liu Fang, Chen Huan-Chun, Liu Zheng-Fei*. Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion. Communications Biology. 2018, 1: 32 (Nature出版社新刊)

7. Ahmed Waqas, Liu Zheng-Fei*. Long non-coding RNAs: novel players in regulation of immune response upon herpesvirus infection. Frontiers in Immunology. 2018, 9:761 (IF= 5.511)

8. Ahmed Waqas, Zheng Ke, Wu Chang-Xian, Liu Zheng-Fei*. Small non-coding RNA AbcR1 address multiple target mRNAs from transcriptional factor and two component response regulator of Brucella melitensis. Jundishapur Journal of Microbiology. 2018, 11(1): e60269 (IF= 1.017)

9. Wu Chang-Xian, Liu Zheng-Fei*. Proteomic profiling of sweat exosome suggests its involvement in skin immunity. Journal of Investigative Dermatology. 2018, 138(1): 89–97 (IF= 6.290)

10. Wang Xin, Wu Chang-Xian, Song Xian-Rong, Chen Huan-Chun, Liu Zheng-Fei *. Comparison of pseudorabies virus China reference strain with emerging variants reveals independent virus evolution within specific geographic regions. Virology. 2017, 506: 92-98 (IF= 3.353)

11. Ahmed Waqas, Zheng Keng and Liu Zheng-Fei*. Small non-coding RNAs: new insights in modulation of host immune response by intracellular bacterial pathogens. Frontiers in Immunology. 2016, 7:431. (IF= 6.429)

12. Ahmed Waqas, Zheng Ke and Liu Zheng-Fei*. Establishment of chronic infection: Brucella’s stealth strategy. Frontiers in Cellular and Infection Microbiology. 2016, 6: 30 (IF=4.3)

13. Wu Chang-Xian#, Xu Xian-Jin#, Zheng Ke, Liu Fang, Yang Xu-Dong, Chen Chuang-Fu, Chen Huan-Chun, Liu Zheng-Fei*. Characterization of ribonuclease III from Brucella. Gene. 2016, 579(2): 183-192. (IF=2.415)

14. Hu Rui-Ming, Zhou Qing, Song Wen-Bo, Sun Er-Chao, Zhang Mei-Mei, He Qi-Gai, Chen Huan-Chun, Wu Bin*, Liu Zheng-Fei*. Novel pseudorabies virus variant with defects in TK, gE and gI protects growing pigs against lethal challenge. Vaccine. 2015, 33(43): 5733-5740. (IF=3.624) （共同通讯作者）

15. Yao Lan#, Wu Chang-Xian#, Zheng Ke, Xu Xian-Jin, Zhang Hui, Chen Chuang-Fu, Liu Zheng-Fei*. Immunogenic response to a recombinant pseudorabies virus carrying bp26 gene of Brucella melitensis in mice. Research in Veterinary Science. 2015, 100: 61-67. (IF=1.511)

16. Tang Qi# , Wu Yi-Quan#, Chen Dong-Sheng, Zhou Qing, Chen Huan-Chun, and Liu Zheng-Fei*. Bovine herpesvirus 5 encodes a unique pattern of microRNAs compared with Bovine herpesvirus 1. Journal of General Virology. 2014,95(3): 671-678. (IF=3.527)

17. Fan Qiang, Yao Lan, Ding Ming, Wang Dan-Jing, Chen Huan-Chun, and Liu Zheng-Fei*. Development of latex agglutination test for rapid detection of antibodies against Bovine herpesvirus 1 and Bovine herpesvirus 5 in cattle. Journal of Veterinary Diagnostic Investigation. 2012, 24(6): 1162-1165. (IF= 1.214)

18. Zheng Ke#, Chen Dong-Sheng#, Wu Yi-Quan, Xu Xian-Jin, Zhang Hui, Chen Chuang-Fu, Chen Huan-Chun, Liu Zheng-Fei*. MicroRNA expression profile in RAW264.7 cells in response to Brucella melitensis infection. International Journal of Biological Sciences. 2012; 8(7): 1013- 1022. (IF= 3.168)

19. Wu Yi-Quan#, Chen Di-Jun#, He Hua-Bin#, Chen Dong-Sheng, Chen Ling-Ling, Chen Huan-Chun, Liu Zheng-Fei*. Pseudorabies virus infected porcine epithelial cell line generates a diverse set of host microRNAs and a special cluster of viral microRNAs. PLoS ONE. 2012, 7(1): e30988. doi:10.1371/journal.pone.0030988. (IF= 4.411)

20. Zhang Hou–Yong# , Chen Dong–Sheng#, Wu Yi–Quan, He Qi–Gai, Chen Huan–Chun, and Liu Zheng–Fei*. Both swine and human cells are capable to support the replication of swine hepatitis E virus type 4 in vitro. Virus Research. 2011, 158: 289-293. (IF= 2.905)

21. Liu ZF, Brum MCS, Doster A, Jones C, and Chowdhury SI. A Bovine herpesvirus type 1 (BHV-1) mutant virus specifying a carboxyl terminal truncation of glycoprotein E (gE) is defective in anterograde neuronal transport in rabbits and calves. Journal of Virology. 2008, 82(15): 7432-7442 (IF= 5.308)

授权发明专利

1. 刘正飞，张美美，陈焕春，王昕。一种伪狂犬病毒LLT区ΔIntron株及构建方法和应用。专利号：ZL 2014 1 0323568.7。证书号第3155677号。授权公告日：2018年11月20日。

2. 刘正飞，陈月平，史晓娜，周云朵。一种犬细小病毒乳胶检测试剂及检测方法和应用。专利号：ZL 201310127883.8。证书号第20130120号。授权公告日: 2016年06月29日。

3. 刘正飞，定明，范强，郭爱珍，高觉婧，陈焕春。牛传染性鼻气管炎ΔTK/ΔgE基因缺失标志活疫苗及制备方法。专利号：ZL 201110043444.X。证书号第2082122号。授权公告日: 2016年05月25日。

4. 刘正飞，商琦，高觉婧，贾贵英，陈焕春。一种表达Nectin-1基因胞外区片段的PK-15细胞系及其构建方法。发明专利号：ZL 2011 1 0203504.X。证书号第1141141号。授权公告日：2013年02月27日。

5. 陈焕春，刘正飞，吴斌，金梅林，方六荣，何启盖。一种伪狂犬病TK-/gE-/gI-基因缺失标志活疫苗及制备方法。发明专利号：ZL03156706.1。授权公告日：2006年3月8日。

备注
  教育部“动物传染病基础与防治技术研究”创新研究群体成员，湖北省“农业动植物病虫害防治的微生物学研究”优秀创新团队骨干成员，中国畜牧兽医学会兽医公共卫生学分会实验室生物安全委员会委员；《亚洲兽医病例研究》编委，《畜牧兽医学报》、Journal of Clinic Microbiology等国内外杂志特邀审稿员。2018年6月主持召开了中英两国青年兽医专家双边论坛。