LI LIN, Dr. & Professor

Academic Position: Professor of Microbiology

Degree: Ph. D. of Science

Phone (office): 027-87286952

E-mail: lilin@mail.hzau.edu.cn

Research field: Functioning genes and biotechnology of Pseudomonas

Education

1995.09-1999.07, Ph. D. of Science, Huazhong Agricultural University, China

1990.09-1993.07, M.D. of Science, Nanjing University, China

1982.09-1986.07, B.D. of Science, Hubei University, China

Work experience 

1993.07-Present,   College of Life Science and Technology, Huazhong Agricultural University, Professor of Microbiology

2019.01-2019.02, College of Biological Sciences, University of California, Davis, Visiting Scholar

2000.07-2002.07, Department of Chemical Engineering, Pohang University of Science and Technology, Republic of Korea, Post-Doctorial Fellow/Visiting Scholar

2003.01-2004.12, Max-Planck-Institut fuer InfektionsBiologie, Germany, Post-Doctorial Fellow

1986.07-1990.09, Department of Chemical Engineering, Hubei University of Arts and Science, Teaching Assistant

Research achievements

Research interests

I have been engaged in fundamental research works on activity characterization, expression regulation, mechanism of action, and primary biotechnological applications of functional genes from Pseudomonas, particularly Pseudomonas syringae and Pseudomonas putida, with respect to nematicidal, pathogenic, and environmentally toxic events. The main interests include: (1) Nematicidal and other functional genes underlying activity characterization, expression regulation and mode of action in P. syringae; (2) Functional genes involving in biodegrading organic chromophoric compounds and synthetic products that are relatively recalcitrant to degradation and significantly threatening the ecosystem, their organizations, metabolic pathways for biodegradation, activity regulation, mechanism of action, etc.; (3) Bacteriogenic Mn oxidation with regard to biomineralization, reactivity of oxides, and biotechnological applications in biodegradation.

General academic achievements

I have devoted to the researches for decades on activity and application of an ice-nucleating gene from P. syringae, activity regulation and action mode of nematicidal genes from various Pseudomonas strains, as well as functional enzymes and active substances in degrading endocrine-disrupting chemicals, organic pesticides, and synthesized dyes from Pseudomonas and Bacillus, with current publications of more than 50 SCI papers and more than 30 other papers, and two Springer published co-authored books, as well as 5 issued Chinese patients.

A list of selected research projects hosted

1. National Natural Science Foundation of China, Genetically modifying Mn-oxidizing a Pseudomonas T34 strain through Type II CRISPA-Cas genome-editing system and biofabrication of lithium-ion batteries using biotemplated strategy (31770108), 2018/12-2021/12, 600,000 RMB.

2. National Natural Science Foundation of China, Molecular mechanism underlying a GntR/HutC-like transcriptional factor (InpR) regulating the pathogenicity of Pseudomonas syringae MB03 in infecting the nematodes (31570123), 2016/01-2019/12, 650,000 RMB, accomplished.

3. National Basic Research Program of China (973 Program), Molecular mechanisms of bacterial pathogenesis to nematodes (2013CB127504), 2013/01-2017/08, 5,9000,000 RMB, accomplished.

4. Public-welfare research project on science and technology of Hubei Province, Research and development of a broad-spectral and highly active biopesticide in Bacillus thuringiensis (2012DBA10001), 2013/01-2014/12, 100,000 RMB, accomplished.

5. National Natural Science Foundation of China, Molecular mechanisms underlying biooxidations of Mn(II) by soil bacteria (31270158), 2013/01-2016/12, 780,000 RMB, accomplished.

6. National Natural Science Foundation of China, Studies on transmembrane and transport effectors in Pseudomonas syringae (31070111), 2011/01-2013/12, 340,000 RMB, accomplished.

7. National High-Tech Research and Development Program (863 Program), Screening of Bacillus thuringiensis highly-insecticidal strains based on omics and metabolic engineering technologies (2008AA02Z112), 2018/01-2010/12, 980,000 RMB, accomplished.

A list of selected publications

1. Anum Bashir, Tian Tian, Xun Yu, Cui Meng, Muhammad Ali, Lin Li*. Pyoverdine-mediated killing of Caenorhabditis elegans by Pseudomonas syringae MB03 and the role of iron in its pathogenicity. International Journal of Molecular Sciences 2020,21:2198. (* Corresponding author)

2. Zhen Zhang, Zhiyong Ruan, Jin Liu, Chang Liu, Fuming Zhang, Robert J. Linhardt, Lin Li*. Complete degradation of bisphenol A and nonylphenol by a composite of biogenic managanese oxides and Escherichia coli cells with surface-displayed multicopper oxidase CotA. Chemical Engineering Journal 2019, 362: 897-908. (* Corresponding author)

3. Zhen Zhang, Jin Liu, Jin Fan, Zhiyong Wang, Lin Li*. Detection of catechol using an electrochemical biosensor based on engineered Escherichia coli cells that surface-display laccase. Analytica Chimica Acta 2018, 1009: 65-72. (* Corresponding author)

4. Abdul Manan, Zahoor Ahmad Bazai, Jin Fan, Huafu Yu, Lin Li*. The Nif3-family protein YqfO03 from Pseudomonas syringae MB03 has multiple nematicidal activities against Caenorhabditis elegans and Meloidogyne incognita. International Journal of Molecular Sciences 2018, 19, 3915. (* Corresponding author)

5. Zhiyong Wang, Jieping Wang, Jin Liu, Hong Chen, Mingshun Li, Lin Li*. Mechanistic insights into manganese oxidation of a soil-borne Mn(II)-oxidizing Escherichia coli strain by global proteomic and genetic analyses. Scientific Reports 2017, 7: 1352. (* Corresponding author)

6．Penggao Hu, Zhongna Zhang, Fei Shen, Xun Yu, Mingshun Li, Hong Ni, Lin Li*. Poly-g-glutamic acid coupled Pseudomonas putida cells surface-displaying metallothioneins: composited copper(II) biosorption and inducible flocculation in aqueous solution. RSC Advances 2017, 7: 18578-18587. (* Corresponding author)

7. Lin Li*, Zhenmin Chen, Ziniu Yu. Mass production, application, and market development of Bacillus thuringiensis biopesticides in China. In: Bacillus thuringiensis and Lysinibacillus sphaericus, Fiuza LM et al (eds.), Springer International Publishing AG 2017. ISBN: 978-3-319-56677-1. pp. 185-212, 2017. DOI: 10.1007/978-3-319-56678-8_12. (* Corresponding author)

8. Xu Qin, Xuemei Xiang, Xiaowen Sun, Hong Ni, Lin Li*. Preparation of nanoscale Bacillus thuringiensis chitinases using silica nanoparticles for nematicide delivery. International Journal of Biological Macromolecules 2016, 82: 13-21. (* Corresponding author)

9. Hua Li, Penggao Hu, Xiuyun Zhao, Ziniu Yu, Lin Li*. Bacillus thuringiensis peptidoglycan hydrolase SleB171 involved in daughter cell separation during cell division. Acta Biochimica et Biophysica Sinica 2016, 48(4): 354-362. (* Corresponding author)

10. Muhammad Ali, Yu Sun, Li Xie, Huafu Yu, Anum Bashir, Lin Li*. The pathogenicity of Pseudomonas syringae MB03 against Caenorhabditis elegans and the transcriptional response of nematicidal genes upon different nutritional conditions. Frontiers in Microbiology 2016, 7: 805. (* Corresponding author)

11. Jin Liu, Luming Tan, Jing Wang, Zhiyong Wang, Hong Ni, Lin Li*. Complete biodegradation of chlorpyrifos by engineered Pseudomonas putida cells expressing surface-immobilized laccases. Chemosphere 2016, 157: 200-207. (* Corresponding author)

12. Zhen Zhang, Zhongming Zhang, Hong Chen, Jin Liu, Chang Liu, Hong Ni, Changsong Zhao, Muhammad Ali, Fan Liu, Lin Li*. Surface Mn(II) oxidation actuated by a multicopper oxidase in a soil bacterium leads to the formation of manganese oxide minerals. Scientific Reports 2015, 5: 10895 (* Corresponding author)

13. Hong Ni, Siquan Zeng, Xu Qin, Xiaowen Sun, Shan Zhang, Xiuyun Zhao, Ziniu Yu, Lin Li*. Molecular docking and site-directed mutagenesis of a Bacillus thuringiensis chitinase to improve chitinolytic, synergistic lepidopteran-larvicidal and nematicidal activities. International Journal of Biological Sciences 2015, 11(3): 304-315. (* Corresponding author)

14．Hong Ni, Zhe Xiong, Ting Ye, Zhen Zhang, Xiaofeng Ma, Lin Li*. Biosorption of copper(II) from aqueous solutions using volcanic rock matrix-immobilized Pseudomonas putida cells with surface-displayed cyanobacterial metallothioneins. Chemical Engineering Journal 2012, 204-205: 264-271. (* Corresponding author)

15. Wei Wang, Zhen Zhang, Hong Ni, Xiaomeng Yang, Qianqian Li, and Lin Li*. Decolorization of industrial synthetic dyes using engineered Pseudomonas putida cells with surface-immobilized bacterial laccase. Microbial Cell Factories 2012, 11: 75. (* Corresponding author)

16. Qianqian Li, Qi Yan, Jinsi Chen, Yan He, Jing Wang, Hongxing Zhang, Ziniu Yu, and Lin Li*. Molecular characterization of an ice nucleation protein variant (InaQ) from Pseudomonas syringae and the analysis of its transmembrane transport activity in Escherichia coli. International Journal of Biological Sciences 2012; 8(8):1097-1108. (* Corresponding author)

17. Xiaohu Shao, Hong Ni, Ting LU, Mengtian Jiang, Hua Li, Xinfeng Huang, Lin Li*. An improved system for the surface immobilisation of proteins on Bacillus thuringiensis vegetative cells and spores through a new spore cortex-lytic enzyme anchor. New Biotechnology 2012, 29(3): 302-310. (* Corresponding author)

18. Lin Li*, Ziniu Yu. Genetically Modified Bacillus thuringiensis Biopesticides. In: Bacillus thuringiensis Biotechnology. Ed. Estibaliz Sansinenea. Springer publishing, 2012. pp. 231-258. ISBN: 978-94-007-3020-5. DOI: 10.1007/978-94-007-3021-2. (* Corresponding author)

Patients issued

1. Li L, Tan LM, Wang ZY, Zhang Z. Preparation technology of biogenic Mn oxides from an Mn(II)-oxidizing Pseudomonas sp. T34 and applications in biodegradation of Ciprofloxacin. China Patient No. : ZL 201410387871.3.

2. Li L, Tang L, Bai ZQ, Liu J. Preparation and application of a biocomposite based on chitosan matrix. China patient no.: 201510195759.4

3. Li L, Xiang XM. Preparation technology and applications of a Bacillus thuringiensis nanoscale biopesticide. China patient no.: ZL 201410189514.6.

4. Li L, Qin X, Sun XW, Gu T, Zhang Z. Screening technology and nematicidal applications of a Pseudomonas syringae. China patient no.: ZL201410199803.4

5. Li L, Bai ZQ, Tang L, Zhang Z. Preparation technology and preparation equipment of chitosan microbeads. China patient no.: ZL 201310741611.7