He Jin
Professional title: Professor
Academic degree: Doctor of Science
E-mail: hejin@mail.hzau.edu.cn
Research areas: Metabolic regulation mechanism of microorganism.
Education Background
09/1985-06/1989: Bachelor of Science in Agriculture (Agricultural Chemistry) at College of Resources and Environment, Huazhong Agricultural University Wuhan, Hubei, China.
09/1991-06/1994: Master of Science in Agriculture (Food Science) at College of Food Science and Technology, Huazhong Agricultural University Wuhan, Hubei, China.
09/1999-06/2003: Doctor of Science (Microbiology) at College of Life Science and Technology, Huazhong Agricultural University Wuhan, Hubei, China.
Work Experience
07/1989-08/1991: Technician, Wuhan Shuangfeng Citric Acid Co., Ltd., Wuhan, Hubei, China.
01/2010-Present: Lecturer, Associate Professor, and Professor (Level 3), College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China.
12/2006-03/2008: Postdoc/Visiting Scholar, Department of Microbiology, University of Illinois at Urbana-Champaign, Illinois, USA.
Scientific Research Achievements
Research directions
Our research interests include microbial functional genomics, the function of bacterial nucleotide second messenger molecules and non-coding RNA, as well as the global regulation of bacterial metabolism. In recent years, the research direction has gradually expanded to include the aspects of synthetic biology and intestinal microorganisms. The main research directions now include: (1) Starting from the basic theoretical research on the nucleotide second messenger molecules of c-di-GMP and c-di-AMP to reveal their regulatory mechanism on the physiological functions of bacteria to lay basis for the application of beneficial microorganisms and prevention and control of harmful microorganisms; (2) Studying the regulatory mechanism of non-coding RNA, such as anti-sense RNA, 6S RNA, and tmRNA, to lay the foundation for the transformation and utilization of bacteria; (3) Developing various synthetic biological regulatory elements; (4) Studying related intestinal microbial flora and colorectal cancer.
Academic achievement
We have long been engaged in microbial functional genomics research and have published more than 60 SCI papers, which have been cited for more than 1500 times in total, with one of which recommended as the F1000Prime paper. In the past 5 years we have focused on the research of the regulatory mechanism of nucleotide second messenger molecules: 1) to systematically reveal the function of c-di-GMP metabolic enzymes in Bacillus thuringiensis; 2) to reveal for the first time the mechanism of how c-di-AMP affects the spores formation of Bacillus thuringiensis; 3) to discover the three tandem riboswitch in nature and its regulatory mechanism; 4) to reveal the regulation mode of c-di-GMP riboswitch as “repression-de-repression”; 5) to reveal the mechanism of c-di-AMP riboswitch in regulating the potassium ion transport in bacteria via the kdpFABC operon.
Presiding research projects
1. National natural science foundation of China General Project. Molecular mechanism of simultaneous regulation of c-di-GMP and c-di-AMP signaling systems by Hfq in Bacillus thuringiensis (31970074), 2020-2023, RMB 580,000.
2. The National Key Research and Development Program of China Subproject. Study on the removing and reducing, migration rules and environmental effects of major pathogenic bacteria during livestock and poultry waste conversion (2018YFD0500204), 2018-2020, RMB 350,000.
3. National natural science foundation of China General Project. A new regulation mechanism of bacterial chemotaxis mediated by c-di-GMP riboswitch Bc1 RNA in Bacillus cereus group strains (31770087), 2018-2021, RMB 550,000.
4. The Fundamental Research Funds for the Central Universities Biomedical research special project. Effect and toxicity of intestinal microbes on regulating neoadjuvant radiotherapy for rectal cancer (2662017PY112), 2017-2019, RMB 350,000. Has been finished.
5. National natural science foundation of China General Project. The structure and functions of TerD, a candidate receptor of cyclic diadenosine monophosphate (c-di-AMP) and its signaling pathway (31270105), 2013-2016, RMB 550,000. Has been finished.
6. “863” Project Subproject. Genetic transformation of a cry5B gene from Bacillus thuringiensis into a Paecilomyces lilacinus genome and the biocontrol activity of the recombinant fungus (2011AA10A205), 2011-2015, RMB 800,000. Has been finished.
7. “973” Project Subproject. Candidate drugs and molecular targets for the regulation of viruses, pathogenic bacteria and fungi, and pests (2010CB126105), 2012-2014, RMB 500,000. Has been finished.
8. National natural science foundation of China General Project. Crystal structure and catalytic mechanism of enoyl-acyl carrier protein reductase (FabV) from Xanthomonas oryzae (31070065), 2011-2013, RMB 330,000. Has been finished.
Representative academic papers (*corresponding author, #joint first author)
1. Jin He*, #, Wen Yin#, Michael Y. Galperin*, Shan-Ho Chou*. Cyclic di-AMP, a second messenger of primary importance: tertiary structures and binding mechanisms. Nucleic Acids Research, 2020, doi: 10.1093/nar/gkaa112.
2. Siyang Xu#, Wen Yin#, Yuling Zhang, Qimei Lv, Yijun Yang, Jin He*. Foes or friends? Bacteria enriched in the tumor microenvironment of colorectal cancer. Cancers, 2020, 12(2): 00372.
3. Cao Zheng, Zhaoqing Yu, Cuiying Du, Yujing Gong, Wen Yin, Xinfeng Li, Zhou Li, Ute Römling, Shan-Ho Chou, Jin He*. 2-Methylcitrate cycle: a well-regulated controller of Bacillus sporulation. Environmental Microbiology, 2020(3): 1125–1140.
4. Xun Wang, Xia Cai, Hongdan Ma, Wen Yin, Li Zhu, Xinfeng Li, Heon M. Lim, Shan-Ho Chou, Jin He*. A c-di-AMP riboswitch controlling kdpFABC operon transcription regulates the potassium transporter system in Bacillus thuringiensis. Communications Biology, 2019, 2:151.
5. Yang Fu, Zhaoqing Yu, Shu Liu, Bo Chen, Li Zhu, Zhou Li, Shan-Ho Chou, Jin He*. C-di-GMP regulates various phenotypes and insecticidal activity of Gram-positive Bacillus thuringiensis. Frontiers in Microbiology, 2018, 9: 0045. (recommended as the F1000Prime paper)
6. Xinfeng Li#, Han Mei#, Fang Chen, Qing Tang, Zhaoqing Yu, Xiaojian Cao, Binda Tembeng Andongma, Shan-Ho Chou, Jin He*. Transcriptome landscape of Mycobacterium smegmatis. Frontiers in Microbiology, 2017, 8: 2505.
7. Maria Kanwal Ali, Xinfeng Li, Qing Tang, Xiaoyu Liu, Fang Chen, Jinfeng Xiao, Muhammad Ali, Shan-Ho Chou, Jin He*. Regulation of inducible potassium transporter KdpFABC by KdpD/KdpE two-component system in Mycobacterium smegmatis. Frontiers in Microbiology, 2017, 8: 570.
8. Hang Zhou#, Cao Zheng#, Jianmei Su#, Bo Chen, Yang Fu, Yuqun Xie, Qing Tang, Shan-Ho Chou, Jin He*. Characterization of a natural triple-tandem c-di-GMP riboswitch and application of the riboswitch-based dual-fluorescence reporter. Scientific Reports, 2016, 6: 20871.
9. Cao Zheng, Yang Ma, Xun Wang, Yuqun Xie, Maria Kanwal Ali, Jin He*. Functional analysis of the sporulation-specific diadenylate cyclase CdaS in Bacillus thuringiensis. Frontiers in Microbiology, 2015, 6: 908.
10. Qing Tang, Yunchao Luo, Cao Zheng, Kang Yin, Maria Kanwal Ali, Xinfeng Li, Jin He*. Functional analysis of a c-di-AMP-specific phosphodiesterase MsPDE from Mycobacterium smegmatis. International Journal of Biological Sciences, 2015, 11(7): 813-824.
11. Qing Tang, Xinfeng Li, Tingting Zou, Huimin Zhang, Yingying Wang, Rongsui Gao, Zhencui Li, Jin He*, Youjun Feng*. Mycobacteria smegmatis BioQ defines a new regulatory network for biotin metabolism. Molecular Microbiology, 2014, 94 (5): 1006-1023.
12. Shumeng Zhang, Jieping Wang, Yale Wei, Qing Tang, Maria Kanwal Ali, Jin He* Heterologous expression of VHb can improve the yield and quality of biocontrol fungus Paecilomyces lilacinus, during submerged fermentation. Journal of Biotechnology, 2014, 187, 147-153.
13. Jianmei Su, Lin Deng, Liangbo Huang, Shujin Guo, Fan Liu and Jin He*. Catalytic oxidation of manganese (II) by multicopper oxidase CueO and characterization of the biogenic Mn oxide. Water Research, 2014, 56, 304-313.
14. Jieping Wang#, Han Mei#, Hongliang Qian, Qing Tang, Xiaocui Liu, Ziniu Yu, Jin He*. Expression profile and regulation of spore and parasporal crystal formation-associated genes in Bacillus thuringiensis. Journal of Proteome Research, 2013, 12(12): 5487-5501.
15. Jieping Wang, Han Mei, Cao Zheng, Hongliang Qian, Cui Cui, Yang Fu, Jianmei Su, Ziduo Liu, Ziniu Yu, Jin He*. The metabolic regulation of Bacillus thuringiensis for the formation of spores and parasporal crystals revealed by the transcriptomics and proteomics. Molecular and Cellular Proteomics, 2013, 12(5): 1363-1376.
Authorized invention patent
1. HE Jin, WEI Yale, WANG Jieping, ZHANG Yan, HAO Bo. Recombinant Paecilomyces lilacinus strain PNVT8 and its application. Chinese inventor's patent, ZL201310406991.9.
Notes
Editorial Board Members of Journals for “Current Bioinformatics”, “Journal of Microbiology” and “Biotic Resourses”.
