Zheng-Fei Liu, Ph.D
Professor of Infectious Animal Disease and Zoonosis，
State Key Laboratory of Agricultural Microbiology,
Department of Preventive Veterinary Medicine,
College of Veterinary Medicine,
Huazhong Agricultural University,
#1 Shizi Shan Street, Hongshan District,
Wuhan, P.R. China 430070
Ph.D. Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, 9/1997 – 6/2002
B.S. Animal Sciences, Huazhong Agricultural University, Wuhan, China, 9/1993 – 6/1997
Postdoctoral Associate, College of Veterinary Medicine, Kansas State University, Manhattan, KS, 10/2004 – 4/2007
Ⅰ. Molecular biology and molecular pathogenisis of animal herpesviruses,such as pseudorabies virus(PRV), Bovine herpesvirus 1(BHV-1) and Bovine herpesvirus 5(BHV-5).
Ⅱ. Intracellular survival and replication of Brucella meltensis.
Ⅲ. Develope vaccines and diagnostic methods for PRV, swine hepatitis E virus(swine HEV), and canine parvovirus 2 (CPV2) .
（1）Yan Kai, Liu Jie, Guan Xiang, Yin Yi-Xin, Peng Hui, Chen Huanchun, and Liu Zheng-Fei*. The carboxyl terminus of tegument protein pUL21 contributes to pseudorabies virus neuroinvasion. Journal of Virology. 2019, 93(7):e02052.1.
（2）Liu Fang, Zheng Ke, Chen Huan-Chun, Liu Zheng-Fei*.Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes. Nucleic Acids Research. 2018, 46(21): e129.
（3）Wu Chang-Xian, Liu Zheng-Fei*. Proteomic profiling of sweat exosome suggests its involvement in skin immunity. Journal of Investigative Dermatology. 2018, 138(1): 89-97.
（4）Zheng Ke, Wang Yang, Li Na, Jiang Fang-Fang, Wu Chang-Xian, Liu Fang, Chen Huan-Chun, Liu Zheng-Fei*. Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion. Communications Biology. 2018, 1: 32.
（5）Ahmed Waqas, Liu Zheng-Fei*. Long non-coding RNAs: novel players in regulation of immune response upon herpesvirus infection. Frontiers in Immunology. 2018, 9:761.
（6）Wang Xin, Zhang Mei-Mei, Yan Kai, Tang Qi, Wu Yi-Quan, He Wen-Bo, Chen Huan-Chun, Liu Zheng-Fei*. The full-length microRNA cluster in the intron of large latency transcript is associated with the virulence of pseudorabies virus. Virology. 2018, 520:59-66.
（7）Guan Xiang, Liu Jie, Jiang Hui, Wu Chang-Xian, Chen Huan-Chun, Liu Zheng-Fei*. Expression of pseudorabies virus-encoded long noncoding RNAs in epithelial cells and neurons. Journal of NeuroVirology. 2018, 24(5):597–605.
（8）Ahmed Waqas, Zheng Ke, Wu Chang-Xian, Liu Zheng-Fei*. Small non-coding RNA AbcR1 address multiple target mRNAs from transcriptional factor and two component response regulator of Brucella melitensis. Jundishapur Journal of Microbiology. 2018, 11(1): e60269.
（9）Wang Xin, Wu Chang-Xian, Song Xian-Rong, Chen Huan-Chun, Liu Zheng-Fei *. Comparison of pseudorabies virus China reference strain with emerging variants reveals independent virus evolution within specific geographic regions. Virology. 2017, 506: 92-98.
（10）Ahmed Waqas, Zheng Keng and Liu Zheng-Fei*. Small non-coding RNAs: new insights in modulation of host immune response by intracellular bacterial pathogens. Frontiers in Immunology. 2016, 7:431.
（11）Ahmed Waqas, Zheng Ke and Liu Zheng-Fei*. Establishment of chronic infection: Brucella’s stealth strategy. Frontiers in Cellular and Infection Microbiology. 2016, 6: 30.
（12）Wu Chang-Xian#, Xu Xian-Jin#, Zheng Ke, Liu Fang, Yang Xu-Dong, Chen Chuang-Fu, Chen Huan-Chun, Liu Zheng-Fei*. Characterization of ribonuclease III from Brucella. Gene. 2016, 579(2): 183-192.
（13）Hu Rui-Ming, Zhou Qing, Song Wen-Bo, Sun Er-Chao, Zhang Mei-Mei, He Qi-Gai, Chen Huan-Chun, Wu Bin*, Liu Zheng-Fei*. Novel pseudorabies virus variant with defects in TK, gE and gI protects growing pigs against lethal challenge. Vaccine. 2015, 33(43): 5733-5740.
（14）Yao Lan#, Wu Chang-Xian#, Zheng Ke, Xu Xian-Jin, Zhang Hui, Chen Chuang-Fu, Liu Zheng-Fei*. Immunogenic response to a recombinant pseudorabies virus carrying bp26 gene of Brucella melitensis in mice. Research in Veterinary Science. 2015, 100: 61-67.
（15）Tang Qi#, Wu Yi-Quan#, Chen Dong-Sheng, Zhou Qing, Chen Huan-Chun, and Liu Zheng-Fei*. Bovine herpesvirus 5 encodes a unique pattern of microRNAs compared with Bovine herpesvirus 1. Journal of General Virology. 2014,95(3): 671-678.
（16）Wu Yi-Quan#, Chen Di-Jun#, He Hua-Bin#, Chen Dong-Sheng, Chen Ling-Ling, Chen Huan-Chun, Liu Zheng-Fei*. Pseudorabies virus infected porcine epithelial cell line generates a diverse set of host microRNAs and a special cluster of viral microRNAs. PLoS ONE. 2012, 7(1): : e30988.
（17）Zheng Ke#, Chen Dong-Sheng#, Wu Yi-Quan, Xu Xian-Jin, Zhang Hui, Chen Chuang-Fu, Chen Huan-Chun, Liu Zheng-Fei*. MicroRNA expression profile in RAW264.7 cells in response to Brucella melitensis infection. International Journal of Biological Sciences. 2012; 8(7): 1013- 1022.
（18）Fan Qiang, Yao Lan, Ding Ming, Wang Dan-Jing, Chen Huan-Chun, and Liu Zheng-Fei*. Development of latex agglutination test for rapid detection of antibodies against Bovine herpesvirus 1 and Bovineherpesvirus 5 in cattle. Journal of Veterinary Diagnostic Investigation.2012, 24(6): 1162-1165.
（19）Zhang Hou–Yong# , Chen Dong–Sheng#, Wu Yi–Quan, He Qi–Gai, Chen Huan–Chun, and Liu Zheng–Fei*. Both swine and human cells are capable to support the replication of swine hepatitis E virus type 4 in vitro. Virus Research. 2011, 158: 289-293.
（20）Liu ZF, Brum MCS, Doster A, Jones C, and Chowdhury SI*. A Bovine herpesvirus type 1 (BHV-1) mutant virus specifying a carboxyl terminal truncation of glycoprotein E (gE) is defective in anterograde neuronal transport in rabbits and calves. Journal of Virology. 2008, 82(15): 7432-7442.