Qigai He, Ph. D.

Professor, College of Veterinary Medicine,

State Key Laboratory of Agricultural Microbiology

Huazhong Agricultural University, Wuhan, China

 

Contact Information

Address: College of Veterinary Medicine, Huazhong Agricultural University

No. 1 Shizishan Street, Hongshan District, Wuhan, P. R. China, 430070

Tel: 86-27-87287288, 87286822

Fax:  86-27-87282608

Email:  he628@mail.hzau.edu.cn

Education

1984.9-1989.6: B.S., Veterinary medicine, The Southwest Nationality University

1990.9-1993.7: M.S., Animal infectious disease and the preventive veterinary medicine, Sichuan Agricultural University

1996.9-2000.6: Ph. D., Animal Genetics and Breeding, Huazhong Agricultural University,

2002.12-2006.03: Postdoc fellowship and visiting scientist, National University of Singapore

2015.4-5: Senior visiting scientist, University of Maryland

Research interests

Aiming to fight against economically important swine diseases, such as Africa swine fever, swine pseudorabies, porcine circovirus-associated disease, porcine epidemic diarrhea, and hemophillus parasuis, our research interests cover the following area:

1. Diagnosis and epidemiological investigation of emerging infectious diseases

2. Mechanism of pathogen pathogenesis through host-pathogen interaction and proteomics

3. New vaccines and diagnostic kit development and application

4. Clinical prevention, control  and eradication of swine diseases

Academic Appointments

1993. 07-1995. 12 Assistant lecture, College of Veterinary Medicine, Huazhong Agricultural University

1996. 01-2000. 12 Lecture, College of Veterinary Medicine, Huazhong Agricultural University

2001. 01-2006. 02 Associate Professor, College of Veterinary Medicine, Huazhong Agricultural University

2006. 03- present  Professor, College of Veterinary Medicine, Huazhong Agricultural University

Professional Society Membership

Fellow Scientist, China Agricultural Research System (CARS)

Board member of Asian Pig Veterinary Society (APVS)

Currently Grants

We have the more than 10 grants from MARA of People’s Republic of China, National Science Foundation of China, Hubei province and Wuhan city as well as those from the Industry, focusing on the identification, pathogenesis mechanism of emerging swine viral diseases, novel diagnostic kit and vaccine development and eradication plan. 

Peer-reviewed publications

1. Yang W, Pin C, Haibing G, Yang C, Hui L, Qigai H. Loop-mediated isothermal amplification targeting the apxIVA gene for detection of Actinobacillus pleuropneumoniae. FEMS Microbiol Lett. 2009 Nov; 300(1):83-9.

2. Yang W, Pin C, Haibing G, Yang C, Hui L, Qigai H. Loop-mediated isothermal amplification targeting the apxIVA gene for detection of Actinobacillus pleuropneumoniae.; FEMS Microbiol Lett. 2009 Nov; 300(1):83-9

3. Wang Yang, Fang Ying, Liu Yingyu, Chen Pin, Li Wentao, Liu Shuqing, Zou Haoyong, He Qigai. Development and evaluation of loop-mediated isothermal amplification for rapid detection of Haemophilus parasuis. FEMS Microbiology Letters, 2010, 313(1):54-60

4. Huimin Zhang, Wentao Li, Zonghai Sheng, Heyou Han and Qigai He. Ultrasensitive detection of porcine circovirus type 2 using gold (III) enhanced chemiluminescence immunoassay. Analyst, 2010,135(7):1680-1685

5. Chen H, Cheng L, Yang S, Liu X, Liu Y, Tang J, Li X, He Q, Zhao S. Molecular characterization, induced expression, and transcriptional regulation of porcine S100A12 gene. Mol Immunol. 2010 Apr; 47(7-8):1601-7.

6. Pin Chen，Yingyu Liu，Chong Liu，Haoyong Zou，Yang Wang，Wentao Li，Dingren Bi and Qigai He. Laboratory Detection of Haemophilus parasuis with Decreased Susceptibility to Nalidixic Acid and Enrofloxacin Due to GyrA and ParC Mutations. Journal of Animal and Veterinary Advances. 2011, 10(21):2870-2873

7. Hao-Yong Zou, Xin-Jun Liu, Feng-Ying Ma, Pin Chen, Rui Zhou, Qi-Gai He. Attenuated Actinobacillus pleuropneumoniae as a bacterial vector for expression of Mycoplasma hyopneumoniae P36 gene. The Journal of Gene Medicine. 2011, 13(4):221-229

8. Zhao Z, Wang C, Xue Y, Tang X, Wu B, Cheng X, He Q, Chen H. The occurrence of Bordetella bronchiseptica in pigs with clinical respiratory disease.Vet J. 2011 Jun; 188(3):337-40.

9. Wang Y, Liu C, Fang Y, Liu X, Li W, Liu S, Liu Y, Liu Y, Charreyre C, Audonnet JC, Chen P, He Q. Transcription analysis on response of porcine alveolar macrophages to Haemophilus parasuis. BMC Genomics. 2012 Feb 13;13:68

10. Cheng S, Zhang M, Li W, Wang Y, Liu Y, He Q; Proteomic analysis of porcine alveolar macrophages infected with porcine circovirus type 2; J Proteomics. 2012 Jun 18;75(11):3258-69

11. Yingyu Liu, Pin Chen, Yang Wang, Wentao Li, Shuang Cheng, Chunmei Wang, Anding Zhang, Qigai He;Transcriptional profiling of Haemophilus parasuis SH0165 response to tilmicosin. Microbial Drug Resist. 2012 Dec;18(6):604-15

12. Liu C, Wang Y, Zhang H, Cheng S, Charreyre C, Audonnet JC, Chen P, He Q. Porcine coronin 1A contributes to nuclear factor-kappa B (NF-κB) inactivation during Haemophilus parasuis infection. PLoS One. 2014 Aug 5; 9(8):e103904.

13. Hu H, Wang C, Guo X, Li W, Wang Y, He Q. Broad activity against porcine bacterial pathogens displayed by two insect antimicrobial peptides moricin and cecropin B. Mol Cells. 2013 Feb; 35(2):106-14

14. Li W, Liu S, Wang Y, Deng F, Yan W, Yang K, Chen H, He Q, Charreyre C, Audoneet JC. Transcription analysis of the porcine alveolar macrophage response to porcine circovirus type 2. BMC Genomics. 2013 May 27; 14:35310.

15. Cheng S, Yan W, Gu W, He Q. The ubiquitin-proteasome system is required for early stages of porcine circovirus type 2 replication. Virology. 2014 May; 456-457:198-204.

16. Chen F, Hu H, Li Z, Huang J, Cai X, Wang C, He Q, et al. Deletion of HAPS_2096 Increases Sensitivity to Cecropin B in Haemophilus parasuis. J Mol Microbiol Biotechnol 2015;25:284-91.

17. Chen F, Zhu Y, Wu M, Ku X, Yao L, He Q. Full-Length Genome Characterization of Chinese Porcine Deltacoronavirus Strain CH/SXD1/2015. Genome Announc 2015;3.

18. Chen F, Zhu Y, Wu M, Ku X, Ye S, Li Z, He Q, et al. Comparative Genomic Analysis of Classical and Variant Virulent Parental/Attenuated Strains of Porcine Epidemic Diarrhea Virus. Viruses 2015;7:5525-38.

19. Li W, Wicht O, van Kuppeveld FJ, He Q, Rottier PJ, Bosch BJ. A Single Point Mutation Creating a Furin Cleavage Site in the Spike Protein Renders Porcine Epidemic Diarrhea Coronavirus Trypsin Independent for Cell Entry and Fusion. J Virol 2015;89:8077-81.

20. Ye S, Li Z, Chen F, Li W, Guo X, Hu H, He Q,et al. Porcine epidemic diarrhea virus ORF3 gene prolongs S-phase, facilitates formation of vesicles and promotes the proliferation of attenuated PEDV. Virus Genes 2015;51:385-92.

21. Ye S, Shao K, Li Z, Guo N, Zuo Y, Li Q, He Q,et al. Antiviral Activity of Graphene Oxide: How Sharp Edged Structure and Charge Matter. ACS Appl Mater Interfaces 2015;7:21571-9.

22. Li, Z., Chen, F., Ye, S., Guo, X., Muhanmmad Memon, A., Wu, M. & He, Q. (2016). Comparative Proteome Analysis of Porcine Jejunum Tissues in Response to a Virulent Strain of Porcine Epidemic Diarrhea Virus and Its Attenuated Strain. Viruses 8, 323.

23. Chen, F., Ku, X., Li, Z. He Q, et al. (2016). Genetic characteristics of porcine epidemic diarrhea virus in Chinese mainland, revealing genetic markers of classical and variant virulent parental/attenuated strains. Gene 588, 95-102.

24. Deng, F., Ye, G., Liu, Q. He Q, et al. (2016). Identification and comparison of receptor binding characteristics of the spike protein of two porcine epidemic diarrhea virus strains. Viruses 8, 55.

25. Guo, X., Hu, H., Chen, F., Li, Z., Ye, S., Cheng, S., Zhang, M. & He, Q. (2016). iTRAQ-based comparative proteomic analysis of Vero cells infected with virulent and CV777 vaccine strain-like strains of porcine epidemic diarrhea virus. Journal of proteomics 130, 65-75.

26. Li, W., van Kuppeveld, F. J., He, Q., Rottier, P. J. & Bosch, B.-J. (2016). Cellular entry of the porcine epidemic diarrhea virus. Virus research 226, 117-127.

27. Guo, X., Zhang, M., Zhang, X. He Q, et al. (2017). Porcine Epidemic Diarrhea Virus Induces Autophagy to Benefit Its Replication. Viruses 9, 53.

28. Li, W., Luo, R., He, Q., van Kuppeveld, F. J., Rottier, P. J. & Bosch, B.-J. (2017). Aminopeptidase N is not required for porcine epidemic diarrhea virus cell entry. Virus research 235, 6-13.

29. Memon AM, Bhuyan AA, Chen F, Guo X, Menghwar H, Zhu Y, Ku X, Chen S, Li Z, He Q. (2017). Development and Validation of Monoclonal Antibody-Based Antigen Capture ELISA for Detection of Group A Porcine Rotavirus.Viral Immunol. 30(4):264-270.

30. Lei Z, Liu Q, Yang B, Ahmed S, Xiong J, Song T, Chen P, Cao J, He Q.(2017).Evaluation of Bioequivalence of Two Long-Acting 20% Oxytetracycline Formulations in Pigs.Front Vet Sci. May 1;4:61.

31. Lei Z, Liu Q, Yang B, Xiong J, Li K, Ahmed S, Hong L, Chen P, He Q, Cao J.(2017). Clinical Efficacy and Residue Depletion of 10% Enrofloxacin Enteric-Coated Granules in Pigs.Front Pharmacol.23;8:294.

32. Zhixin, L. , Qianying, L. , Jincheng, X. , Bing, Y. , Shuaike, Y. , & Qianqian, Z. , Qigai, H. , et al. (2017). Pharmacokinetic and pharmacodynamic evaluation of marbofloxacin and pk/pd modeling against escherichia coli in pigs. Frontiers in Pharmacology, 8, 542-.

33. Liu, S. , Li, W. , Wang, Y. , Gu, C. , Liu, X. , & Charreyre, C. , He Q. ,et al. (2017). Coinfection with haemophilus parasuis serovar 4 increases the virulence of porcine circovirus type 2 in piglets. Virology Journal, 14(1), 227.

34. Zhixin, L. , Qianying, L. , Bing, Y. , Haseeb, K. , Jiyue, C. , & Qigai, H. . (2017). Pk-pd analysis of marbofloxacin against streptococcus suis in pigs. Frontiers in Pharmacology, 8, 856-.

35. Lei, Z. , Liu, Q. , Yang, B. , Ahmed, S. , & He, Q. . (2017). The pharmacokinetic-pharmacodynamic modeling and cut-off values of tildipirosin against haemophilus parasuis. Oncotarget, 9(2), 1673-1690.

36. Zhixin, L. , Qianying, L. , Shuaike, Y. , Bing, Y. , Haseeb, K. , & Kun, L. , Qigai, H. ,et al. (2018). Pk-pd integration modeling and cutoff value of florfenicol against streptococcus suis in pigs. Frontiers in Pharmacology, 9, 2-.

37. Li, Zhonghua, Zeng, Wei, Ye, & Shiyi, He, Qigai, et al. (2018). Cellular hnrnp a1 interacts with nucleocapsid protein of porcine epidemic diarrhea virus and impairs viral replication. Viruses.

38. Zhixin, L. , Qianying, L. , Bing, Y. , Haseeb, K. , Saeed, A. , & Bowen, F. , Qigai, H. , et al. (2018). Evaluation of marbofloxacin in beagle dogs after oral dosing: preclinical safety evaluation and comparative pharmacokinetics of two different tablets. Frontiers in Pharmacology, 9, 306-.

39. Zhixin, L. , Qianying, L. , Qianqian, Z. , Bing, Y. , Haseeb, K. , & Ao, S. , Qigai, H. , et al. (2018). Comparative pharmacokinetics and preliminary pharmacodynamics evaluation of piscidin 1 against prv and pedv in rats. Frontiers in Chemistry, 6, 244-.

40. Lei Z, Liu Q, Qi Y, Yang B, Khaliq H, Xiong J, Moku GK, Ahmed S, Li K, Zhang H, Zhang W, Cao J, He Q. (2018). Optimal regimens and cutoff evaluation of tildipirosin against pasteurella multocida. Frontiers in pharmacology.

41. Guo, N., Zhang, B., Hu, H., Ye, S., Chen, F., Li, Z., ... & He, Q. (2018). Caerin1.1 Suppresses the Growth of Porcine Epidemic Diarrhea Virus In Vitro via Direct Binding to the Virus.. Viruses, 10(9).

42. Zhixin, Lei, Qianying, Liu, Haseeb, & Khaliq, Qigai, He, et al. (2019). Resistant cutoff values and optimal scheme establishments for florfenicol against escherichia coli with pk-pd modeling analysis in pigs. Journal of veterinary pharmacology and therapeutics.

43. Zhang, B., Ku, X., Zhang, X., Zhang, Y., Chen, G., Chen, F., ... & He, Q. (2019). The AI-2/luxS Quorum Sensing System Affects the Growth Characteristics, Biofilm Formation, and Virulence of Haemophilus parasuis. Frontiers in Cellular and Infection Microbiology,.

44. He, D., Chen, F., Ku, X., Yu, X., Li, B., Li, Z., ... & He, Q. (2019). Establishment and application of a multiplex RT-PCR to differentiate wild-type and vaccine strains of porcine epidemic diarrhea virus. Journal of Virological Methods,.

45. Zhang, B., Ku, X., Yu, X., Sun, Q., Wu, H., Chen, F., ... & He, Q. (2019). Prevalence and antimicrobial susceptibilities of bacterial pathogens in Chinese pig farms from 2013 to 2017. Scientific Reports, 9(1).

46. Jiang, Z., Paudyal, N., Xu, Y., Deng, T., Li, F., Pan, H., ... He, Q & Yue, M. (2019). Antibiotic Resistance Profiles of Salmonella Recovered From Finishing Pigs and Slaughter Facilities in Henan, China.. Frontiers in Microbiology,.

47. Hu, H., Guo, N., Chen, S., Guo, X., Liu, X., Ye, S., ... & He, Q. (2019). Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo. Virology Journal, 16(1), 1-10.

48. Li, C., Sun, Y., Li, J., Jiang, C., Zeng, W., Zhang, H., ... & He, Q. (2019). PCV2 Regulates Cellular Inflammatory Responses through Dysregulating Cellular miRNA-mRNA Networks. Viruses, 11(11).

49. Tan Xin, Ku Xugang, Yu Xuexiang, Guo Hengke, He Dongxian, Fan Shengxian, He Qigai. (2017). The IFN-γ-ELISpot for evaluating the immune effect of PRV vaccine. Acta Veterinaria Et Zootechnica Sinica,48(10): 1939-1948.

50.  Li Chang, Ku Xugang, Wang Junwei, Li Jing, Zhu Ling, He Qigai. (2018). Development and Application of a TaqMan-based Real-time Fluorescent PCR for Specific Detection of Porcine Circovirus 3. Acta Veterinaria Et Zootechnica Sinica,49(6): 1320-1326.

51.  Li Chang, Li Jing, He Qigai. (2019). Integrated Analysis of miRNA and mRNA Network of Porcine Circovirus 2 Infecting Porcine Kidney Cell Line. Acta Veterinaria Et Zootechnica Sinica,50(1): 115-125.

52. Wang Junwei, Chen Fangzhou, Ku Xugang, Li chang, He Qigai. (2019). Establishment and Clinical Application of an Indirect ELISA for Detection of Antibodies Against PCV3 Based on Recombinant ORF2 Protein. Acta Veterinaria Et Zootechnica Sinica,50(2): 454-460.