华中农业大学农业微生物学国家重点实验室欢迎您!

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H
何正国
发布人:发布时间:2020-03-06

 

职称 教授

学位 博士

电话 027-87284295

邮箱 hezhengguo@mail.hzau.edu.cn

研究方向 结核分枝杆菌分子生物学

 

教育经历

1988.09-1992.07,华中农业大学微生物学专业,学士

1992.09-1995.07,华中农业大学生命科学技术学院,硕士

1998.09-2001.07,中国科学院微生物研究所,博士


主要职历

2001.07-2004.06,美国哈佛大学医学院生物化学与分子药物系,博士后

2004.08-2005.08,湖北大学生命科学学院,特聘教授

2005. 09-今, 华中农业大学生命科学技术学院,教授(二级岗)

2010.11-今,华中农业大学农业微生物学国家重点实验室,副主任


科研成果

科研方向

主要从事结核分枝杆菌的生长和耐药调控网络及病原-宿主相互作用机制研究。


学术成绩

在国际上率先构建完成了结核分枝杆菌的全局性蛋白质相互作用网络和基因转录调控网络,发现了多种细菌生长和耐药调控新机制。


主持科研项目

1. 十三五国家重点研发计划项目2017-2020,畜禽重要胞内菌基因调控及其与宿主互作的分子机制(2017YFD0500300),2174万元

2. 国家自然科学基金重点项目2018-2022c-di-GMP分子驱动耻垢分枝杆菌抗氧化调控的信号通路与分子机制研究(31730005),291万元

3. 国家自然科学基金重点项目2010-2013,结核分枝杆菌的DNA复制调控网络及其与耐药性突变偶联的遗传机制研究(30930003),180万元

4. 国家杰出青年科学基金2011-2014 微生物生物大分子相互作用(31025002),200万元 

5. 国家973专项课题2006-2010,重要动物病原菌的分子生物学与致病机理的研究--基因组学研究与新基因的发掘(2006CB504402),423万元


代表性学术论文(*通讯作者)

1. Weihui Li, Lihua Hu, Zhiwei Xie, Hui Xu, Meng Li,Tao Cui, Zheng-Guo He* (2018) Cyclic di-GMP integrates functionally divergent transcription factors into a regulation pathway for antioxidant defense. Nucleic Acids Res. 46(14):7270-7283.

2. Weihui Li, Meng Li, Lihua Hu, Jingpeng Zhu, Zhiwei Xie, Jiarui Chen, Zheng-Guo He* (2018) HpoR, a novel c-di-GMP effective transcription factor, links the second messenger’s regulatory function to the mycobacterial antioxidant defense. Nucleic Acids Res. 46(7):3595-3611. 

3. Weihui Li, Tao Cui, Lihua Hu, Ziqing Wang, Zongqiang Li, Zheng-Guo He* (2015) Cyclic diguanylate monophosphate directly binds to human siderocalin and inhibits its antibacterial activity. Nat Commun 6: 8330.

4. Weihui Li, Zheng-Guo He* (2012) LtmA, a novel cyclic di-GMP responsive activator, broadly regulates the expression of lipid transport and metabolism genes in Mycobacterium smegmatis. Nucleic Acids Res. 40(22):11292-30. 

5. Min Yang, Chunhui Gao, Tao Cui, Jingning An, Zheng-Guo He* (2012) A TetR-like regulator broadly affects the expressions of diverse genes in Mycobacterium smegmatis. Nucleic Acids Res. 40(3):1009-1020. 

6. Feng Huang, Zheng-Guo He* (2010) Characterization of an interplay between a Mycobacterium tuberculosis MazF homolog, Rv1495, and its sole DNA topoisomerase I. Nucleic Acids Res. 38(22):8219-8230. 

7. Manman Guo, Hui Feng, Jun Zhang, Wenqin Wang, Yi Wang, Yuqing Li, Chunhui Gao, Huanchun Chen, Ying Feng, Zheng-Guo He* (2009) Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 19(7): 1301-1308. 

8. Lu Zhang, Lei Zhang, Yi Liu, Shifan Yang, Chunhui Gao, Hongchao Gong, Ying Feng, Zheng-Guo He* (2009) Archaeal eukaryote-like Orc1/Cdc6 initiators physically interact with DNA polymerase B1 and regulate its functions. Proc Natl Acad Sci USA. 106(19):7792-7797. 

9. Yu Liu, Hongyang Wang, Tao Cui, Xiling Zhou, Yanxia Jia, Hua Zhang*, Zheng-Guo He* (2016) NapM, a new nucleoid-associated protein, broadly regulates gene expression and affects mycobacterial resistance to anti-tuberculosis drugs. Mol Microbiol. 101(1): 167-181.

10. Yi Wang, Tao Cui, Cong Zhang, Min Yang, Yuanxia Huang, Weihui Li, Lei Zhang, Chunhui Gao, Yang He, Yuqing Li, Feng Huang, Jumei Zeng, Cheng Huang, Qiong Yang, Yuxi Tian, Chunchao Zhao, Huanchun Chen, Hua Zhang, Zheng-Guo He* (2010) Global protein-protein interaction network in the human pathogen Mycobacterium tuberculosis H37Rv. J Proteome Res. 9(12): 6665 - 6677.

11. Yuqing Li, Jumei Zeng, Jianfang Shi, Mingchao Wang, Muding Rao, Chaolun Xue, Yanli Du, Zheng-Guo He* (2010) A proteome-scale identification of novel antigenic proteins in Mycobacterium tuberculosis towards diagnostic and vaccine development. J Proteome Res. 9(9): 4812 – 4822. 

12. Jumei Zeng,Tao Cui, Zheng-Guo He* (2012) A Genome-wide Regulator-DNA Interaction Network in the human pathogen Mycobacterium tuberculosis H37Rv. J Proteome Res. 11(9), 4682-4692. 

13. Hu Q, Zhang J, Chen Y, Hu L, Li W, Zheng-Guo He* (2019). Cyclic di-GMP co-activates the two-component transcriptional regulator DevR in Mycobacterium smegmatis in response to oxidative stress. J Biol Chem. 294(34):12729-12742.

14. Chen Zhu, Yu Liu, Lihua Hu, Min Yang*, Zheng-Guo He* (2018) Molecular mechanism of the synergistic activity of ethambutol and isoniazid against Mycobacterium tuberculosis. J Biol Chem. 293(43):16741-16750. 

15. Lei Liu, Cheng Huang, Zheng-Guo He*. (2014)A TetR-family transcriptional factor directly regulates the expression of a 3-methyladenine DNA glycosylase and physically interacts with the enzyme to stimulate its base excision activity in Mycobacterium bovis BCG. J Biol Chem. 289(13):9065-9075. 

16. Shifan Yang, Zengqiang Gao, Tingting Li, Min Yang, Tianyi Zhang, Yuhui Dong*, Zheng-Guo He* (2013) Structural basis for interaction between M. smegmatis Ms6564, a TetR family master regulator, and its target DNA. J Biol Chem. 288(33):23687-95. 

17. Lei Zhang, Zheng-Guo He* (2013) Radiation-sensitive gene A (RadA) targets DisA, DNA integrity scanning protein A, to negatively affect its cyclic-di-AMP synthesis activity in Mycobacterium smegmatis. J Biol Chem. 288(31):22426-22436. 

18. Lei Zhang, Weihui Li, Zheng-Guo He* (2013) DarR, a TetR-like transcriptional factor, is a cyclic-di-AMP responsive repressor in Mycobacterium smegmatis. J Biol Chem. 288 (5): 3085-3096. 

19. Muding Rao, Huicong Liu, Min Yang, Chunchao zhao, Zheng-Guo He* (2012) A copper-responsive global repressor regulates expression of diverse membrane-associated transporters and bacterial drug resistance in mycobacteria. J Biol Chem. 287(47):39271-39371.

20. Yi Wang, Yuanxia Huang, Chaolun Xue, Yang He, Zheng-Guo He* (2011) A ClpR-like regulator specifically recognizes a RecA-independent promoter motif and broadly regulates expression of DNA damage inducible genes in mycobacteria. J Biol Chem. 286(36): 31159-31167. 


备注

国家杰出青年基金获得者,国家高层次人才特殊支持计划科技创新领军人才

 

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