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H
何启盖
发布人:发布时间:2020-03-17

职称 教授

学位 博士

政治面貌 中共党员

电话 027-87287288, 87286822

邮箱 he628@mail.hzau.edu.cn

研究方向 动物传染病诊断与防控


教育经历

1984.09-1989.07,西南民族大学,兽医/本科

1990.09-1993.07,四川农业大学,传染病学与预防兽医学/硕士

1996.09-2000.07,华中农业大学,动物遗传育种与繁殖/博士


主要职历

1993.07-2002.11华中农业大学,助教讲师/副教授

2002.12-2006.03新加坡国立大学,访问学者/博士后

2006.04-现在,华中农业大学动科-动医学院,教授

(期间:2015.4.25-2015.05:美国马里兰大学,高级访问学者)


科研成果

科研方向:主要从事动物传染病防控技术研究与产品研制,主要侧重在:

1. 动物传染病学流行病学调查与致病机理

2. 动物新生物制品研究与应用

3. 动物传染病诊断与净化


学术成绩

1. 聚焦新发传染病防控的应用基础研究

(1)伪狂犬病:分离鉴定了猪伪狂犬病变异毒株,研究其致病机理;研究了伪狂犬病毒作为活疫苗载体的特性,研制了伪狂犬病gE-ELISA血清学鉴别诊断试剂盒,是市面上唯一的国产试剂盒;研制了猪伪狂犬病新毒株灭活疫苗。为猪伪狂犬病防控提供了新技术与新产品。

(2)猪病毒性腹泻:持续开展分子流行病学监测,分离鉴定了与猪腹泻有关的猪流行性腹泻病毒和猪轮状病毒,分析了新毒株毒力增强的机制,研制了IgA-ELISA试剂盒,用于评价黏膜抗体。

(3)猪圆环病毒病:分离鉴定猪圆环病毒2型,以组学技术研究其免疫抑制机理研究;进而研制了灭活疫苗,获得新兽药注册证书,应用于国内养殖场;监测到猪圆环病毒3型的流行。

2. 围绕猪场细菌病流行现状,重点开展副猪嗜血杆菌病流行病学监控、病原分离鉴定,构建不同突变株,研究了其致病机制。

3. 以具有资质的诊断实验室为依托,为国内外猪场提供诊断与防控技术服务。承担政府委托的疫情监测任务。

2008年获得湖北省科技进步一等奖,2012年参与获得国家科技进步一等奖(第5),2016年获得国家科技进步二等奖(第3)。本人2019年获农业农村部农牧渔业丰收奖(贡献奖)。制定了2项国家标准。


主持科研项目

1. 国家现代农业(生猪)产业技术体系课题(CARS-35CARS-362007-2020 840万元

2. 国家自然科学基金面上项目-猪流行性腹泻病毒变异毒株感染性克隆构建与分子致病机制研究(31272572),2013/01-2016/1289万元

3. 家重点研发计划课题-猪重要疫病抗体快速检测技术研究2016YFD0500702, 2016-2020150万元

4. 国家自然科学基金面上项目,内质网应激在猪流行性腹泻病毒诱导细胞自噬中的作用与生物学效应(31972667),2020/01-2023/1256万元(直接经费)

5. 国家自然科学基金应急专项(参加)-非洲猪瘟病毒流行特征与演化及早期检测技术(31941004,2019.07-2022.0690万元。

6. 湖北省技术创新专项-非洲猪瘟防控关键技术研究与示范(2019ABA089),2019/01-2021/1230万元。

7. 湖北省国际合作课题-新型猪伪狂犬病毒的变异特征和防控技术(2018AHB011),2018-201930万元。

8.武汉市国际合作课题-猪流行性腹泻新型疫苗和鉴别诊断方法的研制与临床应用(2016030409020215,2016-201820万元。

9. 企业横向合作课题(若干。基于保密性原则,省略)。


代表性学术论文

1. Yang W, Pin C, Haibing G, Yang C, Hui L, Qigai H. Loop-mediated isothermal amplification targeting the apxIVA gene for detection of Actinobacillus pleuropneumoniae. FEMS Microbiol Lett. 2009 Nov; 300(1):83-9.

2. Yang W, Pin C, Haibing G, Yang C, Hui L, Qigai H. Loop-mediated isothermal amplification targeting the apxIVA gene for detection of Actinobacillus pleuropneumoniae.; FEMS Microbiol Lett. 2009 Nov; 300(1):83-9

3. Wang Yang, Fang Ying, Liu Yingyu, Chen Pin, Li Wentao, Liu Shuqing, Zou Haoyong, He Qigai. Development and evaluation of loop-mediated isothermal amplification for rapid detection of Haemophilus parasuis. FEMS Microbiology Letters, 2010, 313(1):54-60

4. Huimin Zhang, Wentao Li, Zonghai Sheng, Heyou Han and Qigai He. Ultrasensitive detection of porcine circovirus type 2 using gold (III) enhanced chemiluminescence immunoassay. Analyst, 2010,135(7):1680-1685

5. Chen H, Cheng L, Yang S, Liu X, Liu Y, Tang J, Li X, He Q, Zhao S. Molecular characterization, induced expression, and transcriptional regulation of porcine S100A12 gene. Mol Immunol. 2010 Apr; 47(7-8):1601-7.

6. Pin ChenYingyu LiuChong LiuHaoyong ZouYang WangWentao LiDingren Bi and Qigai He. Laboratory Detection of Haemophilus parasuis with Decreased Susceptibility to Nalidixic Acid and Enrofloxacin Due to GyrA and ParC Mutations. Journal of Animal and Veterinary Advances. 2011, 10(21):2870-2873

7. Hao-Yong Zou, Xin-Jun Liu, Feng-Ying Ma, Pin Chen, Rui Zhou, Qi-Gai He. Attenuated Actinobacillus pleuropneumoniae as a bacterial vector for expression of Mycoplasma hyopneumoniae P36 gene. The Journal of Gene Medicine. 2011, 13(4):221-229

8. Zhao Z, Wang C, Xue Y, Tang X, Wu B, Cheng X, He Q, Chen H. The occurrence of Bordetella bronchiseptica in pigs with clinical respiratory disease.Vet J. 2011 Jun; 188(3):337-40.

9. Wang Y, Liu C, Fang Y, Liu X, Li W, Liu S, Liu Y, Liu Y, Charreyre C, Audonnet JC, Chen P, He Q. Transcription analysis on response of porcine alveolar macrophages to Haemophilus parasuis. BMC Genomics. 2012 Feb 13;13:68

10. Cheng S, Zhang M, Li W, Wang Y, Liu Y, He Q; Proteomic analysis of porcine alveolar macrophages infected with porcine circovirus type 2; J Proteomics. 2012 Jun 18;75(11):3258-69

11. Yingyu Liu, Pin Chen, Yang Wang, Wentao Li, Shuang Cheng, Chunmei Wang, Anding Zhang, Qigai He;Transcriptional profiling of Haemophilus parasuis SH0165 response to tilmicosin. Microbial Drug Resist. 2012 Dec;18(6):604-15

12. Liu C, Wang Y, Zhang H, Cheng S, Charreyre C, Audonnet JC, Chen P, He Q. Porcine coronin 1A contributes to nuclear factor-kappa B (NF-κB) inactivation during Haemophilus parasuis infection. PLoS One. 2014 Aug 5; 9(8):e103904.

13. Hu H, Wang C, Guo X, Li W, Wang Y, He Q. Broad activity against porcine bacterial pathogens displayed by two insect antimicrobial peptides moricin and cecropin B. Mol Cells. 2013 Feb; 35(2):106-14

14. Li W, Liu S, Wang Y, Deng F, Yan W, Yang K, Chen H, He Q, Charreyre C, Audoneet JC. Transcription analysis of the porcine alveolar macrophage response to porcine circovirus type 2. BMC Genomics. 2013 May 27; 14:35310.

15. Cheng S, Yan W, Gu W, He Q. The ubiquitin-proteasome system is required for early stages of porcine circovirus type 2 replication. Virology. 2014 May; 456-457:198-204.

16. Chen F, Hu H, Li Z, Huang J, Cai X, Wang C, He Q, et al. Deletion of HAPS_2096 Increases Sensitivity to Cecropin B in Haemophilus parasuis. J Mol Microbiol Biotechnol 2015;25:284-91.

17. Chen F, Zhu Y, Wu M, Ku X, Yao L, He Q. Full-Length Genome Characterization of Chinese Porcine Deltacoronavirus Strain CH/SXD1/2015. Genome Announc 2015;3.

18. Chen F, Zhu Y, Wu M, Ku X, Ye S, Li Z, He Q, et al. Comparative Genomic Analysis of Classical and Variant Virulent Parental/Attenuated Strains of Porcine Epidemic Diarrhea Virus. Viruses 2015;7:5525-38.

19. Li W, Wicht O, van Kuppeveld FJ, He Q, Rottier PJ, Bosch BJ. A Single Point Mutation Creating a Furin Cleavage Site in the Spike Protein Renders Porcine Epidemic Diarrhea Coronavirus Trypsin Independent for Cell Entry and Fusion. J Virol 2015;89:8077-81.

20. Ye S, Li Z, Chen F, Li W, Guo X, Hu H, He Q,et al. Porcine epidemic diarrhea virus ORF3 gene prolongs S-phase, facilitates formation of vesicles and promotes the proliferation of attenuated PEDV. Virus Genes 2015;51:385-92.

21. Ye S, Shao K, Li Z, Guo N, Zuo Y, Li Q, He Q,et al. Antiviral Activity of Graphene Oxide: How Sharp Edged Structure and Charge Matter. ACS Appl Mater Interfaces 2015;7:21571-9.

22. Li, Z., Chen, F., Ye, S., Guo, X., Muhanmmad Memon, A., Wu, M. & He, Q. (2016). Comparative Proteome Analysis of Porcine Jejunum Tissues in Response to a Virulent Strain of Porcine Epidemic Diarrhea Virus and Its Attenuated Strain. Viruses 8, 323.

23. Chen, F., Ku, X., Li, Z. He Q, et al. (2016). Genetic characteristics of porcine epidemic diarrhea virus in Chinese mainland, revealing genetic markers of classical and variant virulent parental/attenuated strains. Gene 588, 95-102.

24. Deng, F., Ye, G., Liu, Q. He Q, et al. (2016). Identification and comparison of receptor binding characteristics of the spike protein of two porcine epidemic diarrhea virus strains. Viruses 8, 55.

25. Guo, X., Hu, H., Chen, F., Li, Z., Ye, S., Cheng, S., Zhang, M. & He, Q. (2016). iTRAQ-based comparative proteomic analysis of Vero cells infected with virulent and CV777 vaccine strain-like strains of porcine epidemic diarrhea virus. Journal of proteomics 130, 65-75.

26. Li, W., van Kuppeveld, F. J., He, Q., Rottier, P. J. & Bosch, B.-J. (2016). Cellular entry of the porcine epidemic diarrhea virus. Virus research 226, 117-127.

27. Guo, X., Zhang, M., Zhang, X. He Q, et al. (2017). Porcine Epidemic Diarrhea Virus Induces Autophagy to Benefit Its Replication. Viruses 9, 53.

28. Li, W., Luo, R., He, Q., van Kuppeveld, F. J., Rottier, P. J. & Bosch, B.-J. (2017). Aminopeptidase N is not required for porcine epidemic diarrhea virus cell entry. Virus research 235, 6-13.

29. Memon AM, Bhuyan AA, Chen F, Guo X, Menghwar H, Zhu Y, Ku X, Chen S, Li Z, He Q. (2017). Development and Validation of Monoclonal Antibody-Based Antigen Capture ELISA for Detection of Group A Porcine Rotavirus.Viral Immunol. 30(4):264-270.

30. Lei Z, Liu Q, Yang B, Ahmed S, Xiong J, Song T, Chen P, Cao J, He Q.(2017).Evaluation of Bioequivalence of Two Long-Acting 20% Oxytetracycline Formulations in Pigs.Front Vet Sci. May 1;4:61.

31. Lei Z, Liu Q, Yang B, Xiong J, Li K, Ahmed S, Hong L, Chen P, He Q, Cao J.(2017). Clinical Efficacy and Residue Depletion of 10% Enrofloxacin Enteric-Coated Granules in Pigs.Front Pharmacol.23;8:294.

32. Zhixin, L. , Qianying, L. , Jincheng, X. , Bing, Y. , Shuaike, Y. , & Qianqian, Z. , Qigai, H. , et al. (2017). Pharmacokinetic and pharmacodynamic evaluation of marbofloxacin and pk/pd modeling against escherichia coli in pigs. Frontiers in Pharmacology, 8, 542-.

33. Liu, S. , Li, W. , Wang, Y. , Gu, C. , Liu, X. , & Charreyre, C. , He Q. ,et al. (2017). Coinfection with haemophilus parasuis serovar 4 increases the virulence of porcine circovirus type 2 in piglets. Virology Journal, 14(1), 227.

34. Zhixin, L. , Qianying, L. , Bing, Y. , Haseeb, K. , Jiyue, C. , & Qigai, H. . (2017). Pk-pd analysis of marbofloxacin against streptococcus suis in pigs. Frontiers in Pharmacology, 8, 856-.

35. Lei, Z. , Liu, Q. , Yang, B. , Ahmed, S. , & He, Q. . (2017). The pharmacokinetic-pharmacodynamic modeling and cut-off values of tildipirosin against haemophilus parasuis. Oncotarget, 9(2), 1673-1690.

36. Zhixin, L. , Qianying, L. , Shuaike, Y. , Bing, Y. , Haseeb, K. , & Kun, L. , Qigai, H. ,et al. (2018). Pk-pd integration modeling and cutoff value of florfenicol against streptococcus suis in pigs. Frontiers in Pharmacology, 9, 2-.

37. Li, Zhonghua, Zeng, Wei, Ye, & Shiyi, He, Qigai, et al. (2018). Cellular hnrnp a1 interacts with nucleocapsid protein of porcine epidemic diarrhea virus and impairs viral replication. Viruses.

38. Zhixin, L. , Qianying, L. , Bing, Y. , Haseeb, K. , Saeed, A. , & Bowen, F. , Qigai, H. , et al. (2018). Evaluation of marbofloxacin in beagle dogs after oral dosing: preclinical safety evaluation and comparative pharmacokinetics of two different tablets. Frontiers in Pharmacology, 9, 306-.

39. Zhixin, L. , Qianying, L. , Qianqian, Z. , Bing, Y. , Haseeb, K. , & Ao, S. , Qigai, H. , et al. (2018). Comparative pharmacokinetics and preliminary pharmacodynamics evaluation of piscidin 1 against prv and pedv in rats. Frontiers in Chemistry, 6, 244-.

40. Lei Z, Liu Q, Qi Y, Yang B, Khaliq H, Xiong J, Moku GK, Ahmed S, Li K, Zhang H, Zhang W, Cao J, He Q. (2018). Optimal regimens and cutoff evaluation of tildipirosin against pasteurella multocida. Frontiers in pharmacology.

41. Guo, N., Zhang, B., Hu, H., Ye, S., Chen, F., Li, Z., ... & He, Q. (2018). Caerin1.1 Suppresses the Growth of Porcine Epidemic Diarrhea Virus In Vitro via Direct Binding to the Virus.. Viruses, 10(9).

42. Zhixin, Lei, Qianying, Liu, Haseeb, & Khaliq, Qigai, He, et al. (2019). Resistant cutoff values and optimal scheme establishments for florfenicol against escherichia coli with pk-pd modeling analysis in pigs. Journal of veterinary pharmacology and therapeutics.

43. Zhang, B., Ku, X., Zhang, X., Zhang, Y., Chen, G., Chen, F., ... & He, Q. (2019). The AI-2/luxS Quorum Sensing System Affects the Growth Characteristics, Biofilm Formation, and Virulence of Haemophilus parasuis. Frontiers in Cellular and Infection Microbiology,.

44. He, D., Chen, F., Ku, X., Yu, X., Li, B., Li, Z., ... & He, Q. (2019). Establishment and application of a multiplex RT-PCR to differentiate wild-type and vaccine strains of porcine epidemic diarrhea virus. Journal of Virological Methods,.

45. Zhang, B., Ku, X., Yu, X., Sun, Q., Wu, H., Chen, F., ... & He, Q. (2019). Prevalence and antimicrobial susceptibilities of bacterial pathogens in Chinese pig farms from 2013 to 2017. Scientific Reports, 9(1).

46. Jiang, Z., Paudyal, N., Xu, Y., Deng, T., Li, F., Pan, H., ... He, Q & Yue, M. (2019). Antibiotic Resistance Profiles of Salmonella Recovered From Finishing Pigs and Slaughter Facilities in Henan, China.. Frontiers in Microbiology,.

47. Hu, H., Guo, N., Chen, S., Guo, X., Liu, X., Ye, S., ... & He, Q. (2019). Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo. Virology Journal, 16(1), 1-10.

48. Li, C., Sun, Y., Li, J., Jiang, C., Zeng, W., Zhang, H., ... & He, Q. (2019). PCV2 Regulates Cellular Inflammatory Responses through Dysregulating Cellular miRNA-mRNA Networks. Viruses, 11(11).

49. 谭鑫,库旭钢,于学祥,郭恒科,何东贤,范盛先,何启盖. (2017). IFN-γ-ELISpot方法用于评价猪伪狂犬病疫苗免疫效果. 畜牧兽医学报, 48(10): 1939-1948.

50. 李畅, 库旭钢, 王俊伟, 李静, 朱玲, 何启盖. (2018). 猪圆环病毒3型TaqMan荧光定量PCR方法的建立和初步应用. 畜牧兽医学报, 49(6): 1320-1326.

51. 李畅, 李静, 何启盖. (2019). 猪圆环病毒2型感染PK-15细胞差异表达miRNA和mRNA互作网络. 畜牧兽医学报, 50(1): 115-125.

52. 王俊伟, 陈芳洲, 库旭钢, 李畅, 何启盖. (2019). 基于PCV3-Cap蛋白间接ELISA检测方法的建立及临床应用. 畜牧兽医学报, 50(2): 454-460.



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