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郭爱珍
发布人:发布时间:2020-03-31


职称 教授

学位 博士

政治面貌 中共党员

电话 027-87286861

邮箱 aizhen@mail.hzau.edu.cn

研究方向 兽医流行病学和病原学、致病机理和免疫机理、新型疫苗和诊断技术及其产品的研发


教育经历

1981.09-1985.06,湖南农学院,牧医系兽医专业毕业,农学学士

1985.09-1988.06 南京农业大学,动物医学院兽医病理专业毕业,农学硕士

1995.09-1998.06 南京农业大学,预防兽医学专业毕业,农学博士


主要职历 

1988.07 –1995.09,在南京奶业集团南京乳牛科学研究所工作,任兽医师与科研培训办公室主任,从事奶牛疾病防制、乳及乳制品质量控制、非蛋白氮饲料开发等领域的研究、管理与推广应用工作。1993.8-12 在荷兰国际农业中心培训奶业管理。

1998.07-1999.11 , 南京农业大学动物医学院任讲师,从事兽医微生物学与免疫学领域的教学与科研及推广应用工作。

1999.12-2003.02, 南京农业大学动物医学院任副教授,硕士导师。从事兽医微生物学与免疫学领域的教学与科研及推广应用工作。所开本科生课程有兽医病毒学”“病毒学”“动物保护概论”“鱼病学等,研究生课程有病毒学实验技术。另任研究生支部书记与研究生辅导员。

2002.01-2003.02, 在美国宾夕法尼亚大学兽医学院微生物学实验室做博士后,研究课题为鼠沙门氏菌1型菌毛与鼠伤寒树突状细胞相互作用的研究。

2003.3-至今,华中农业大学动物医学院工作,华中农业大学农业微生物学国家重点实验室固定人员,200311月晋升为教授,2004年被聘为博士生导师。从事牛传染病及人兽共患传染病的科研、教学与技术推广与咨询服务工作。


科研成果 

科研方向

主要从事牛传染病和人兽共患病防控理论和技术研究,研究领域涉及兽医流行病学和病原学、致病机理和免疫机理、新型疫苗和诊断技术及其产品的研发等,研究的主要病原有:牛分支杆菌、牛支原体、牛传染性鼻气管炎病毒、牛病毒性腹泻病毒、沙门氏菌等。


学术成绩

从事动物结核病和牛传染病防控相关的基础和应用研究。近五年来,先后主持了公益性行业(农业)科研专项(首席)、“973”课题、国家自然科学基金面上和国际合作重大项目等20余个科研项目;发表论文84篇,其中SCI论文35篇;出版专著16部,其中主编《牛结核病》等相关专著6部;获授权专利8项(含结核病相关专利4项);制定国家标准3项和地方标准7项(含结核病检测技术国家标准2项和地方标准2项);主持完成的人与动物结核病新型特异诊断试剂等获湖北省技术发明二等奖等省级奖2项和武汉市科技进步一等奖1项。研发了牛结核病诊断试剂盒等多种试剂盒及多种新型牛病疫苗,获新兽药注册证书(二类)3个。产品转化给6家生物制品企业,共主办了九届全国牛病防制研讨会,组织技术培训50余次,促进了牛病防控技术的推广和应用。


主持科研项目

1.基于奶样检测对奶牛布鲁氏菌病和结核病实施风险预测的研究,湖北省技术创新专项(对外科技合作类),项目编号:2019AHB069,2019.1.12020.12.31

2.环二核苷酸磷酸二酯酶调控牛支原体生长特性的分子机制研究。国家自然基金面上项目(项目号:31772745)。2018.1~2021.12,主持。

3.牛支原体和丝状支原体丝状亚种小菌落型分泌蛋白组学比较研究。 国家自然科学基金国际(地区)合作交流项目(项目号:31661143015)。2017.12021.12,主持。

4.基于全基因组的ncRNA 序列分析发掘结核分枝杆菌和巨噬细胞的互作机制。国家自然基金面上项目(项目号:31472219)。2015.12018.12,主持。

5.牛疱疹病毒1型UL51基因功能的研究。教育部高校博士点基金(项目号:20130146110003)。2014.1.12016.12.31,主持。

6.神农架金丝猴人工种群疾病防控与健康监测关键技术与应用。国家科技支撑计划课题(课题号:2013BAD03B01-02)。2013.1.12017.12.31,主持。

7.牛结核检测试剂盒的产业化转化和推广。农业科技成果转化资金项目(项目号:2012GB23600628),2012.32014.3,主持。

8.牛支原体新型毒力相关基因的鉴定和功能研究。国家自然科学基金面上项目(项目号:31272587),2013.012016.12,主持。

9.病原菌在动物和人中的流行与传播机制,973课题(课题号:2012CB518801),2012.12016.8,主持。

10.结核病分子标识的筛选、验证和结核病诊断研究。艾滋病和病毒性肝炎等重大传染病防治科技重大专项课题(课题号:2012ZX10003002-016),20122016,主持。

11.家畜生产运输过程中环境应激响应及调控技术研究. 农业公益性行业科研专项(项目号:201003060), 20102014,项目首席。


代表性学术论文

1. Muhammad Zubair, Farhan Anwar Khan, Harish Menghwar, Muhammad Faisal, Muhammad Ashraf, Muhammad Asif Rasheed, Marawan A Marawan, Ali Dawood1, Yingyu Chen, Huanchun Chen,Aizhen Guo.Progresses on bacterial secretomes enlighten research on Mycoplasma secretome.Microbial Pathogenesis,Available online 17 March 2020, 104160

2. Muhammad Zubair, Shafii Abdullahi Muhamed, Farhan Anwar Khan, Gang Zhao, Harish Menghwar, Muhammad Faisal, Hui Zhang, Xifang Zhu,Muhammad Asif Rasheed, Yingyu Chen, Marawan Adel, Huanchun Chen,Aizhen Guo.Identification of 60 secretory proteins for Mycoplasma bovis with secretome assay.Microbial Pathogenesis,2020, in press.

3. Sohail Raza, Farzana Shahin, Wenjun Zhai, Hanxiong Li, Gualtiero Alvisi, Kui Yang*, Xi Chen, Yingyu Chen, Jianguo Chen, Changmin Hu, Huanchun Chen, Aizhen Guo.Ivermectin Inhibits Bovine Herpesvirus 1 DNA Polymerase Nuclear Import and Interferes with Viral Replication.Microorganisms 2020, 8, 409.

4. Zhu X, Dong Y, Baranowski E, Li X, Zhao G, Hao Z, Zhang H, Chen Y, Hu C, Chen H, Citti C, Guo A. Mbov_0503 Encodes a Novel Cytoadhesin that Facilitates Mycoplasma bovis Interaction with Tight Junctions. Microorganisms. 2020, 8(2). pii: E164.

5. Zhu X, Dordet-Frisoni E, Gillard L, Ba A, Hygonenq MC, Sagné E, Nouvel LX,Maillard R, Assié S, Guo A, Citti C, Baranowski E. Extracellular DNA: A Nutritional Trigger of Mycoplasma bovis Cytotoxicity. Front Microbiol. 2019, 10:2753.

6. Liu Han, Xiong Xuekai, Zhai Wenjun, Zhu Tingting, Zhu Xiaojie, Zhu Yifan, Peng Yongchong, Wang Jieru, Chen Huanchun, Chen Yingyu, Guo Aizhen*. Upregulation of Cytokines and Differentiation of Th17 and Treg by Dendritic Cells: Central Role of Prostaglandin E2 Induced by Mycobacterium bovis. Microorganisms2020, 8(2), pii: E195.

7. Yifan Zhu, Yao Xiao, Delai Kong, Han Liu, Xi Chen, Yingyu Chen, Tingting Zhu, Yongchong Peng, Wenjun Zhai, Changmin Hu, Huanchun Chen, Sizhu Suolang, Aizhen Guo* and Jiaqiang Niu*. Down-regulation of miR-378d increased Rab10 expression to help clearance of Mycobacterium tuberculosis in macrophages. Frontiers Cellular and Infection. Microbiology,2020,10,doi: 10.3389/fcimb.2020.00108.

8. Chao J, Han X, Liu K, Li Q, Peng Q, Lu S, Zhao G, Zhu X, Hu G, Dong Y, Hu C, ChenY, Chen J, Chen H, Guo A. Pathogenesis and Immunity Associated with Pathogenic Th17 and Th1 as Revealed by Differential Transcription Profiles of Peripheral Blood Mononuclear Cells of Calves Induced by Virulent and Attenuated Mycoplasma bovis Strains in vivo. Genes, 2019, 10(9): 656. DOI:10.3390/genes10090656.

9. Anwar Khan F , Chen X, Shoaib M, Shah M, Ahmad F, Khan H, Ur Rehman F, Saeed M, Ahmad I, Ahmad I, Guo A. Two dimensional gel electrophoresis (2-DE) for high-throughput proteome analyses of Mycoplasma bovis. Acta Biochim Pol. 2019, 66(3):321-327.

10. Qingye Zhuang, Suchun Wang, Shuo Liu, Guangyu Hou, Jinping Li, Wenming Jiang, Kaicheng Wang,Cheng Peng, Dong Liu, Aizhen Guo* and Jiming Chen*.Diversity and distribution of type A influenza viruses: an updated panorama analysis based on protein sequences.Virology Journal,(2019)16:85

11. Qiao Hongxing, Zhang Xiaojing, Shi Hongtao, Song Yuzhen, Bian Chuanzhou, Guo Aizhen*.Assessment of the physicochemical properties and bacterial composition of Lactobacillus plantarum and Enterococcus faecium-fermented Astragalus membranaceus using single molecule, real-time sequencing technology. SCIENTIFIC REPORTS. 2018,8:11862.(if=4.122)

12. Xi Chen, Jing Huang, Hongmei Zhu, Yongpeng Guo, Farhan Anwar Khan,Harish Menghwar, Gang Zhao, Aizhen Guo*. P27 (MBOV_RS03440) is a novel fibronectin binding adhesin of Mycoplasma bovis. International Journal of Medical Microbiology. 308 (2018) 848–857.

13. Ren Ningning, Jinli Jingfang, Chen Yingyu*, Zhou Xia, Wang Jieru, Ge Pan, Farhan Anwar Khan, Zhang Li, Hu Changmin, Ian D. Robertson, Chen Huanchun, Guo Aizhen*.Identification of new diagnostic biomarkers for Mycobacterium tuberculosis and the potential application in the serodiagnosis of human tuberculosis. Microbial Biotechnology 2018,11(5):893-904.

14. Jieru Wang, Xiaojie Zhu, Xuekai Xiong, Pan Ge, Han Liu, Ningning Ren, Farhan Anwar Khan, Xia Zhou,Li Zhang, Xu Yuan, Xi Chen, Yingyu Chen, Changmin Hu, Ian D. Robertson, Huanchun Chen and Aizhen Guo.Identification of potential urine proteins and microRNA biomarkers for the diagnosis of pulmonary tuberculosis patients. Emerging Microbes & Infections. (2018) 7:63

15. Farzana Shahin, Sohail Raza, Kui Yang, Changmin Hu, Yingyu Chen, Huanchun Chen, Aizhen Guo?. Bovine herpesvirus 1 tegument protein UL21 plays critical roles in viral secondary envelopment and cell-to-cell spreading, Oncotarget, 2017,8(55): 94462-94480.

16. Farhan Anwar Khan, Gang Zhao, Yusi Guo, Muhammad Faisal,Jin Chao,Xi Chen, Chenfei He, Harish Menghwar, Rahim Dad, Muhammad Zubair, Changmin Hu, Yingyu Chen, Huanchun Chen, Zhang Rui, Aizhen Guo?. Proteomics identification and characterization of MbovP730 as a potential DIVA antigen of Mycoplasma bovis, Oncotarget, 2017,1: 1-15.

17. Xuekai Xiong, Rui Wang, Dachuan Deng, Yingyu Chen, Han Liu, Tianqi Wang, Jieru Wang, Xiaojie Zhu, Xifang Zhu, Yongqiang Zhu, Xinyan Lu, Huanchun Chen, Huajun Zheng, Aizhen Guo. Comparative Genomics of a Bovine Mycobacterium tuberculosis Isolate and Other Strains Reveals Its Potential Mechanism of Bovine Adaptation. Frontiers in Microbiology, 12, 2017, doi:10.3389 /fmicb.2017. 02500.

18. Menghwar H, He C,Zhang H,Zhao G,Zhu X,Khan FA, Faisal M, Rasheed MA, Zubair M, Memon AM, Ridley A, Robertson ID, Chen Y, Guo A*. Genotype distribution of Chinese Mycoplasma bovis isolates and their evolutionary relationship to strains from other countries. Microb Pathog. 2017, 111: 108-117.

19. Flahou B, Rossi M, Bakker J, Langermans JA, Heuvelman E, Solnick JV, Martin ME, O'Rourke J, Ngoan LD, Hoa NX, Nakamura M, Øverby A, Matsui H, Ota H, Matsumoto T, Foss DL, Kopta LA, Omotosho O, Franciosini MP, Casagrande Proietti P, Guo A, Liu H, Borilova G, Bracarense AP, Lindén SK, De Bruyckere S, Zhang G, De Witte C, Smet A, Pasmans F, Ducatelle R, Corander J, Haesebrouck F. Evidence for a primate origin of zoonotic Helicobacter suis colonizing domesticated pigs. ISME J. 2017 Sep 8.

20. Xia Miaomiao, Lia Zheng, Chen Yingyu, Xiang Yuqiang, Yang Li, Zhao Xiangwei, Guo Aizhen, Chen Huanchun, Tan Chen*, Hu Yonggang*. Protein self-assembly via Zr4+ions on spore-based microspheres for immunoassays. Sensors and Actuators B: Chemical.2018, 254:166-176.

21. Rasheed MA, Qi J, Zhu X, Chenfei H, Menghwar H, Khan FA, Zhao G, Zubair M, Hu C, Chen Y, Chen H, Guo A. Comparative Genomics of Mycoplasma bovis Strains Reveals That Decreased Virulence with Increasing Passages Might Correlate with Potential Virulence-Related Factors. Front Cell Infect Microbiol. 2017, 7: 177.

22. Zhao G, Zhang H, Chen X, Zhu X, Guo Y, He C, Anwar Khan F, Chen Y, Hu C, Chen H, Guo A. Mycoplasma bovis NADH oxidase functions as both a NADH oxidizing and O(2) reducing enzyme and an adhesin. Sci Rep. 2017, 7(1): 44.

23. Chen Y, Wang J, Ge P, Cao D, Miao B, Robertson I, Zhou X, Zhang L, Chen H, Guo A. Tissue inhibitor of metalloproteinases 1, a novel biomarker of tuberculosis. Mol Med Rep. 2017, 15(1): 483-487. 

24. Khan FA, Faisal M, Chao J, Liu K, Chen X, Zhao G, Menghwar H, Zhang H, Zhu X, Rasheed MA, He C, Hu C, Chen Y, Baranowski E, Chen H, Guo A. Immunoproteomic identification of MbovP579, a promising diagnostic biomarker for serological detection of Mycoplasma bovis infection. Oncotarget. 2016, 7(26): 39376-39395.

25. Zhang H, Zhao G, Guo Y, Menghwar H, Chen Y, Chen H, Guo A. Mycoplasma bovis MBOV_RS02825 encodes a putative virulence-related nuclease.  Int J Mol Sci, 2016, 17(5): 628.

26. Raza S, Deng M, Shahin F, Yang K, Hu C, Chen Y, Chen H, Guo A. A bovine herpesvirus 1 pUL51 deletion mutant shows impaired viral growth in vitro and reduced virulence in rabbits. Oncotarget. 2016,7(11):12235-12253.

27. Deng M, Ji S, Fei W, Raza S, He C, Chen Y, Chen H, Guo A. Prevalence study and genetic typing of bovine viral diarrhea virus (BVDV) in four bovine species in China. PLoS One. 2015, 10(4): e0121718. 

28. Han X, Khan FA, Zhu X, Zhang R, Mustafa R, Hu C, Chen Y, Chen H, Guo A. Establishment of an antibody avidity test to differentiate vaccinated cattle from those naturally infected with Mycoplasma bovis. Vet J. 2015, 203(1): 79-84.

29. Zhang R, Han XX, Mustafa R, Qi JJ, Chen X, Hu CM, Chen YY, Chen HC, Guo AZ. Attenuated Mycoplasma bovis strains provide protection against virulent infection in calves. Vaccine, 2014, 32(25):3107-3114.

30. Chen Y, Wu J, Tu L, Xiong X, Hu X, Huang J, Xu Z, Zhang X, Hu C, Hu X, Guo A,Wang Y, Chen H.1H-NMR spectroscopy revealed abnormal serum metabolic profile of cattle infected with Mycobacterium tuberculosis. PlosOne, 2013, 8(9): e74507. 

31. Zhang X, Li S, Luo Y, Chen Y* , Cheng S, Zhang G, Hu C, Chen H, Guo A*.Mycobacterium bovis and BCG induce different patterns of cytokine and chemokine production in dendritic cells and differentiation patterns in CD4+ T cells. Microbiology-SGM. 2013, 159(Pt2): 366-379.

32. Qi J, Guo A, Cui P, Chen Y, Mustafa R, Ba X, Hu C, Bai Z, Chen X, Shi L, Chen H. Comparative Geno-Plasticity Analysis of Mycoplasma bovis HB0801 (Chinese Isolate). PLoS One. 2012,7(5): e38239. 


授权发明专利

1.郭爱珍,晋丛,刘晗,陈颖钰,胡长敏,陈焕春。缺失phoQrpoS基因的都柏林沙门氏菌及应用。申请号:201510110209.8。发文序号:。申请日:2015-03-13。主分类号 C12N 1/20。授权日:2018.3.9,专利号:ZL201510110209.8

2.郭爱珍,赵刚,陈曦,汪乾坤,张慧,郭雨丝,陈颖钰,胡长敏。一株牛支原体NADH 氧化酶的单克隆抗体。专利申请号:201510054798.2,发文序号:2015020301078670,申请日期:2015203日。专利号:ZL201510054798.2,授权日:2018.3.9

3.郭爱珍,任泽民,陈颖钰,胡长敏,张瑞,崔鹏,陈焕春.一种牛支原体单克隆抗体及制备方法和应用。专利申请号:ZL201210201726.22012-6-19申请。授权日:2015.04.8,发文序号:2015020200339190

4.郭爱珍;姜勇;陈颖钰;张策;郝丽影;邹新峰;陈焕春。一种猕猴IFN-γ的单克隆抗体杂交瘤细胞及制备方法和应用。专利申请号:ZL201210098394.X2012-4-6申请。授权日:2015-04-8,发文序号201502020300850110

5.郭爱珍,刘晓乐,张敏敏,陈颖玉,胡长敏,陈焕春. 一种用于快速诊断牛副流感病毒3型的RT-PCR检测试剂盒。ZL201110122089.5。申请日:2011-5-12;授权日:2013-2-27。已停。

6.郭爱珍,刘颖,张广智,廖娟红,刘冬光,于清龙,王冰,邹新峰,熊家军,杨利国,陈焕春.梅花鹿γ-干扰素双抗体夹心ELISA检测方法及试剂盒与应用.专利号:ZL201010104575.X,专利申请日:20100203日,授权公告日:20130306日。

7.郭爱珍,张敏敏,刘正飞,付书林,陈焕春。一种缺失gGTK基因的重组牛传染性鼻气管炎病毒和应用。申请号:200910273357.6。申请日:2009-12-24。专利号:ZL200910273357.6,授权公告日:2012-7-25

8.郭爱珍,白智迪,巴晓亮,胡长敏,陈颖钰,彭清洁,张瑞,祁晶晶,崔朋,陈焕春.一种牛支原体致弱菌株及其应用。申请号:201110117094.7。申请日:2011.5.6专利号:ZL201110117094.7,授权日:2012-10-3

9.郭爱珍,曹莎,陈焕春,刘子铎,张承才,谭亚娣.显性抑制突变体F427D作为炭疽芽孢杆菌毒素抑制剂及疫苗的应用.申请日:2008.11.7。专利号:ZL200810197553.5,授权公告日:2012-6-27

10.郭爱珍, 白智迪, 石磊, 胡长敏, 彭清洁, 陈焕春, 巴小亮, 任泽民. 牛支原体环介导等温扩增检测方法. 专利号:ZL200910272782.3, 授权日2012-06-07. 20090097(专利序号)发明专利。申请日2009-11-18,(专利授权号),发文序号2012040600129260

11.郭爱珍,曹莎,陈焕春,刘子铎,张承才,谭亚娣. 显性抑制突变体F427N作为炭疽毒素抑制剂及疫苗的应用. 申请日:2008.11.7.专利号:ZL200810197554.X. 授权日:2011-1-26.

12.郭爱珍,刘冬光,曹莎,陈颖钰,廖娟红,于清龙,凌洁玉,陈焕春.三基因融合的重组牛结核特异性抗原蛋白及制备方法。申请号:2009.3.2,专利号ZL200910060913.1。授权日:2011-1-262014-11恢复专利权力。

13.郭爱珍,张书环,陈焕春,谭亚娣,晁彦杰,陈颖钰,钦博,吕文,匡有吉。检测牛结核抗体的免疫胶体金试纸条及制备方法。申请日:2006.12.30,专利号:ZL200610166551.0;授权日:2009-6-3

14.郭爱珍,彭博,陈焕春,陈汉阳,金梅林,王冲。含有与鸡传染性支气管炎病毒特异性结合短肽的噬菌体及应用,申请日:2004.7.5,专利授权号(ZL200410009301.7,授权日:2008-03-12


备注

郭爱珍,女,湖南沅江人。华中农业大学二级岗教授,中共党员,美国宾夕法尼亚大学博士后,硕士生及博士生导师。澳大利亚默多克大学客座教授,农业微生物学国家重点实验室固定人员。国务院政府特殊津贴获得者,全国农业科研杰出人才和牛病防控创新团队带头人,现代农业(肉牛牦牛)产业技术体系疾病控制研究室主任及岗位专家,国家动物结核病专业实验室(武汉)主任,湖北省兽医流行病学国际科技合作基地副主任。从事动物结核病和牛传染病防控相关的基础和应用研究。先后主持了公益性行业(农业)科研专项(首席)、“973”课题、国家自然科学基金面上和国际合作重大项目等20余个科研项目;近五年来,发表论文77篇,其中SCI论文37篇。出版专著16部,其中主编《牛结核病》等相关专著6部;获授权专利8项;制定国家标准3项和地方标准7项;主持完成的人与动物结核病新型特异诊断试剂等获湖北省技术发明二等奖等省级奖2项和武汉市科技进步一等奖1项。研发了牛结核病诊断试剂盒等多种试剂盒及多种新型牛病疫苗,获新兽药注册证书(二类)3个。产品转化给6家生物制品企业,促进了牛病防控技术的推广和应用。


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